Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Nutrients. 2021 Sep 9;13(9):3141. doi: 10.3390/nu13093141.
High-fat diet (HFD) consumption induced gut dysbiosis, inflammation, obese-insulin resistance. Perilla seed oil (PSO) is a rich source of omega-3 polyunsaturated fatty acids with health promotional effects. However, the effects of PSO on gut microbiota/inflammation and metabolic disturbance in HFD-induced obesity have not been investigated. Therefore, we aimed to compare the effects of different doses of PSO and metformin on gut microbiota/inflammation, and metabolic parameters in HFD-fed rats.
Thirty-six male Wistar rats were fed either a normal diet or an HFD for 24 weeks. At week 13, HFD-fed rats received either 50, 100, and 500 mg/kg/day of PSO or 300 mg/kg/day metformin for 12 weeks. After 24 weeks, the metabolic parameters, gut microbiota, gut barrier, inflammation, and oxidative stress were determined.
HFD-fed rats showed gut dysbiosis, gut barrier disruption with inflammation, increased oxidative stress, metabolic endotoxemia, and insulin resistance. Treatment with PSO and metformin not only effectively attenuated gut dysbiosis, but also improved gut barrier integrity and decreased gut inflammation. PSO also decreased oxidative stress, metabolic endotoxemia, and insulin resistance in HFD-fed rats. Metformin had greater benefits than PSO.
PSO and metformin had the beneficial effect on attenuating gut inflammation and metabolic disturbance in obese-insulin resistance.
高脂肪饮食（HFD）会导致肠道菌群失调、炎症和肥胖型胰岛素抵抗。紫苏籽油（PSO）是一种富含ω-3 多不饱和脂肪酸的食物，具有促进健康的作用。然而，PSO 对 HFD 诱导肥胖中肠道菌群/炎症和代谢紊乱的影响尚未得到研究。因此，我们旨在比较不同剂量的 PSO 和二甲双胍对 HFD 喂养大鼠肠道菌群/炎症和代谢参数的影响。
36 只雄性 Wistar 大鼠分别喂食正常饮食或 HFD 24 周。在第 13 周，HFD 喂养的大鼠接受 50、100 和 500mg/kg/天的 PSO 或 300mg/kg/天的二甲双胍治疗 12 周。24 周后，测定代谢参数、肠道菌群、肠道屏障、炎症和氧化应激。
HFD 喂养的大鼠表现出肠道菌群失调、肠道屏障破坏伴炎症、氧化应激增加、代谢性内毒素血症和胰岛素抵抗。PSO 和二甲双胍治疗不仅有效减轻了肠道菌群失调，还改善了肠道屏障的完整性，减少了肠道炎症。PSO 还降低了 HFD 喂养大鼠的氧化应激、代谢性内毒素血症和胰岛素抵抗。二甲双胍的效果优于 PSO。
PSO 和二甲双胍对减轻肥胖型胰岛素抵抗大鼠的肠道炎症和代谢紊乱具有有益作用。
