Department of Food Science and Human Nutrition, Jeonbuk National University, Jeonju, Republic of Korea.
Obesity Research Center, Jeonbuk National University, Jeonju, Republic of Korea.
J Med Food. 2020 Aug;23(8):818-826. doi: 10.1089/jmf.2019.4675. Epub 2020 Jun 17.
This study aimed to evaluate the effect of perilla oil (PO) on high-fat diet (HD)-induced colonic inflammation. Male C57BL/6J mice (5 weeks old) were divided into four groups: normal diet, HD, HD supplemented with fish oil (FO), and HD supplemented with PO, and were fed experimental diets for 16 weeks. PO significantly ameliorated ( < .05) the HD-induced colon inflammation as indicated by the increased colon length and low macroscopic score. PO increased the number of and reduced the number of , which in turn resulted in the lowering of endotoxin levels. Proinflammatory cytokines in serum and colon such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α were also decreased by PO treatment. In addition, PO suppressed the expression of cyclooxygenase 2 and inducible nitric oxide, and inhibited the activation of nuclear factor-κB in the colon while increasing the expression of the tight junction protein, Zonula occludens-1. The gene expression of , a membrane receptor activated by omega-3 fatty acids, was increased in the oil-treated groups. Altogether, PO improved HD-induced colon inflammatory conditions, and the effects were similar to those of FO, confirming that PO is a potential omega-3 fatty acid source for dietary supplements.
本研究旨在评估紫苏油（PO）对高脂肪饮食（HD）诱导的结肠炎症的影响。雄性 C57BL/6J 小鼠（5 周龄）分为四组：正常饮食组、HD 组、HD 加鱼油（FO）组和 HD 加 PO 组，并分别用实验饮食喂养 16 周。PO 显著改善了（<0.05）HD 诱导的结肠炎症，表现为结肠长度增加和宏观评分降低。PO 增加了 的数量，减少了 的数量，从而降低了内毒素水平。血清和结肠中的促炎细胞因子，如白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子-α，也被 PO 治疗降低。此外，PO 抑制了结肠中环氧化酶 2 和诱导型一氧化氮合酶的表达，抑制了核因子-κB 的激活，同时增加了紧密连接蛋白 Zonula occludens-1 的表达。一种膜受体的基因表达，该受体被 ω-3 脂肪酸激活，在油处理组中增加。总之，PO 改善了 HD 诱导的结肠炎症状态，其效果与 FO 相似，证实 PO 是膳食补充剂中潜在的 ω-3 脂肪酸来源。
