Zhang Chiran, Yang Qianhui, Zhang Xiaoqin, Zhang Xian, Yu Tongyuan, Wu Yuhuan, Fang Yunxia, Xue Dawei
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Plants (Basel). 2021 Sep 6;10(9):1849. doi: 10.3390/plants10091849.
In recent years, cadmium (Cd) pollution in soil has increased with increasing industrial activities, which has restricted crop growth and agricultural development. The heavy metal ATPase () gene family contributes to heavy metal stress resistance in plants. In this study, 21 genes () were identified in barley ( L., ) using bioinformatics methods. Based on phylogenetic analysis and domain distribution, barley genes were divided into five groups (A-E), and complete analyses were performed in terms of physicochemical properties, structural characteristics, conserved domains, and chromosome localization. The expression pattern analysis showed that most genes were expressed in barley and exhibited tissue specificity. According to the fragments per kilobase of exon per million fragments values in shoots from seedlings at the 10 cm shoot stage (LEA) and phylogenetic analysis, five genes were selected for expression analysis under Cd stress. Among the five genes, three (, , and ) were upregulated and two ( and ) were downregulated following Cd treatments. This study serves as a foundation for clarifying the functions of HvHMA proteins in the heavy metal stress resistance of barley.
近年来，随着工业活动的增加，土壤中的镉（Cd）污染加剧，这限制了作物生长和农业发展。重金属ATP酶（）基因家族有助于植物抵抗重金属胁迫。本研究利用生物信息学方法在大麦（L.，）中鉴定出21个基因（）。基于系统发育分析和结构域分布，大麦基因被分为五组（A - E），并对其理化性质、结构特征、保守结构域和染色体定位进行了全面分析。表达模式分析表明，大多数基因在大麦中表达并表现出组织特异性。根据10厘米苗龄幼苗地上部分每百万片段中外显子每千碱基的片段数（LEA）值和系统发育分析，选择了五个基因进行镉胁迫下的表达分析。在这五个基因中，镉处理后三个（、和）基因上调，两个（和）基因下调。本研究为阐明HvHMA蛋白在大麦重金属胁迫抗性中的功能奠定了基础。
