Zavala-Gómez Carlos Eduardo, Rodríguez-deLeón Eloy, Bah Mamadou Moustapha, Feregrino-Pérez Ana Angélica, Campos-Guillén Juan, Amaro-Reyes Aldo, Rodríguez-Morales José Alberto, García-Trejo Juan Fernando, Flores-Macias Antonio, Figueroa-Brito Rodolfo, Ramos-López Miguel Angel
Biosystems Engineering Group, Division of Graduate Studies, Faculty of Engineering, Autonomous University of Queretaro, Cerro de las Campanas, S/N, Colonia Las Campanas, C.U., Santiago de Querétaro 76010, Mexico.
Faculty of Chemistry, Autonomous University of Querétaro, Cerro de las Campanas, S/N, Colonia Las Campanas, Santiago de Querétaro 76010, Mexico.
Plants (Basel). 2021 Sep 14;10(9):1902. doi: 10.3390/plants10091902.
Castor bean () seeds contain ricinine, an alkaloid with insecticidal and insectistatic activities. Elicitation with salicylic acid (SA) has proven to stress and might modify the ricinine concentration. The aim of this study was to evaluate the concentration of ricinine in the bagasse of seeds from elicited with exogenous SA under greenhouse conditions. Plants were grown and divided into five groups, which were sprayed with SA and drench with 50 mL 60 days after sowing with concentrations of SA (0, 100, 300, 600 and 900 µM). Clusters were mixed and separated according to the treatment, and dried. The seeds were ground, the oil was extracted by Soxhlet with hexane, and then the bagasse was extracted with methanol. Ricinine was determined by HPLC. Elicitation did not change the plant height or diameter; the control group had 9.17 µg mL of ricinine; and the concentrations followed a hormesis curve with the peak at 300 µM of SA that had a ricinine concentration of 18.25 µg mL. Elicitation with SA might be a cost-effective technique to increase ricinine from bagasse.
蓖麻籽含有蓖麻碱,一种具有杀虫和抑虫活性的生物碱。水杨酸(SA)诱导已被证明会造成胁迫并可能改变蓖麻碱浓度。本研究的目的是评估在温室条件下用外源SA诱导的蓖麻籽粕中蓖麻碱的浓度。将植株种植并分为五组,在播种60天后分别用浓度为0、100、300、600和900μM的SA进行喷雾和50 mL灌根处理。根据处理将果穗混合并分开,然后干燥。将种子研磨,用己烷通过索氏提取法提取油,然后用甲醇提取粕。通过高效液相色谱法测定蓖麻碱。诱导并未改变植株高度或直径;对照组的蓖麻碱含量为9.17μg/mL;浓度呈剂量效应曲线,在300μM SA时达到峰值,此时蓖麻碱浓度为18.25μg/mL。用水杨酸诱导可能是一种从蓖麻籽粕中提高蓖麻碱含量的经济有效的技术。