Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, Japan.
Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, Japan.
Mater Sci Eng C Mater Biol Appl. 2021 Oct;129:112377. doi: 10.1016/j.msec.2021.112377. Epub 2021 Aug 14.
The immunomodulatory antibacterial activity and osteoimmunomodulatory properties of implantable biomaterials significantly influence bone regeneration. Various types of ultraviolet (UV) instrument are currently in use to greatly enhance the antibacterial activity and osteoconductive capability of titanium, it remains unclear how UV treatment modulates immune response. Compared to traditional UV treatment, the combination of low-dose ozone with UV irradiation is considered a new option to give benefits to surface modification and reduce the drawbacks of UV and ozone individually. Herein, the aim of this study was to elucidate the immune-modulatory properties of macrophages on UV/ozone-irradiated titanium that serve as defense against S. aureus and the crosstalk between immune cells and osteoblasts. Three different cell and bacteria co-culture systems were developed in order to investigate the race between host cells and bacteria to occupy the surface. In vitro immunological experiments indicated that UV/ozone irradiation significantly enhanced the phagocytic and bactericidal activity of macrophages against S. aureus. Further, in vitro and in vivo studies evidenced the favorable osteoimmune environment for osteogenic differentiation and bone formation. This research suggests vital therapeutic potential of UV/ozone irradiation for preventing the biomaterial-associated infections and achieving favorable bone formation simultaneously.
植入生物材料的免疫调节抗菌活性和骨免疫调节特性对骨再生有重要影响。目前有各种类型的紫外线（UV）仪器被用于极大地增强钛的抗菌活性和骨传导能力，但紫外线处理如何调节免疫反应仍不清楚。与传统的紫外线处理相比，低浓度臭氧与紫外线照射的结合被认为是一种新的选择，可以使表面改性受益，并减少紫外线和臭氧各自的缺点。本研究旨在阐明作为防御金黄色葡萄球菌的手段，经紫外线/臭氧辐照的钛的巨噬细胞的免疫调节特性，以及免疫细胞和成骨细胞之间的串扰。为了研究宿主细胞和细菌争夺表面的竞争，开发了三种不同的细胞和细菌共培养系统。体外免疫学实验表明，紫外线/臭氧辐照显著增强了巨噬细胞对金黄色葡萄球菌的吞噬和杀菌活性。此外，体外和体内研究证实了有利于成骨分化和骨形成的骨免疫环境。这项研究表明，紫外线/臭氧辐照具有重要的治疗潜力，可同时预防生物材料相关感染和获得良好的骨形成。
