Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells, Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Adv Exp Med Biol. 2021;1345:17-33. doi: 10.1007/978-3-030-82735-9_3.
Lung transplantation may be considered as a final treatment option for diseases such as chronic lung disease, pulmonary hypertension, bronchopulmonary dysplasia, pulmonary fibrosis, and end-stage lung disease. The five-year survival rate of lung transplants is nearly 50%. Unfortunately, many patients will die before a suitable lung donor can be found. Importantly, the shortage of donor organs has been a significant problem in lung transplantation. The tissue engineering approach uses de- and recellularization of lung tissue to create functional lung substitutes to overcome donor lung limitations. Decellularization is hope for generating an intact ECM in the development of the engineered lung. The goal of decellularization is to prepare a suitable scaffold of lung tissue that contains an appropriate framework for the functionality of regenerated lung tissue. In this chapter, we aim to describe the decellularization protocols for lung tissue regenerative purposes.
肺移植可能被视为治疗慢性肺部疾病、肺动脉高压、支气管肺发育不良、肺纤维化和终末期肺病等疾病的最终治疗选择。肺移植的五年存活率接近 50%。不幸的是，许多患者在找到合适的肺供体之前就已经死亡。重要的是，供体器官短缺一直是肺移植中的一个重大问题。组织工程方法使用肺组织的去细胞化和再细胞化来创建功能性肺替代物，以克服供体肺的限制。去细胞化有望在工程肺的发展中产生完整的细胞外基质。去细胞化的目标是准备合适的肺组织支架，其中包含再生肺组织功能的适当框架。在本章中，我们旨在描述用于肺组织再生目的的去细胞化方案。
