Peterson C, Goldman J E
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2758-62. doi: 10.1073/pnas.83.8.2758.
Aging and Alzheimer disease lead to alterations in several biochemical properties of cultured skin fibroblasts. Total bound calcium increases in fibroblasts due to normal aging (+52%) and is elevated even further with Alzheimer disease (+197%). Processes that require mitochondrial function, such as glucose and glutamine oxidation, declined in cells from aged donors (-25%) and decreased even further in Alzheimer disease (-46%). In addition, biosynthetic processes that depend upon mitochondrial function, such as glucose or glutamine incorporation into protein and lipid, paralleled the oxidative decreases. Cytosolic and nuclear processes such as leucine incorporation into protein and thymidine into DNA were depressed more by aging than Alzheimer disease. These findings suggest that calcium homeostasis and mitochondrial functions are altered more by Alzheimer disease than normal aging.
衰老和阿尔茨海默病会导致培养的皮肤成纤维细胞的多种生化特性发生改变。由于正常衰老，成纤维细胞中总结合钙增加（+52%），而在阿尔茨海默病患者中进一步升高（+197%）。需要线粒体功能的过程，如葡萄糖和谷氨酰胺氧化，在老年供体的细胞中下降（-25%），在阿尔茨海默病患者中下降得更明显（-46%）。此外，依赖线粒体功能的生物合成过程，如葡萄糖或谷氨酰胺掺入蛋白质和脂质中，与氧化作用的下降情况相似。细胞溶质和细胞核过程，如亮氨酸掺入蛋白质和胸苷掺入DNA，衰老比阿尔茨海默病的抑制作用更强。这些发现表明，与正常衰老相比，阿尔茨海默病对钙稳态和线粒体功能的改变更大。
