Feng Chen, He Chengming, Wang Yifan, Xu Hehan, Xu Keheng, Zhao Yu, Yao Bowen, Zhang Yinhe, Zhao Yan, Idrice Carther Kue Foka, Luo Jun, Sun DaQian, Gao Hongtao, Wang Fawei, Li Xiaowei, Liu Weican, Dong Yuanyuan, Wang Nan, Zhou Yonggang, Li Haiyan
College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, 130118, China.
College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, 130118, China; College of Tropical Crops, Hainan University, Haikou, 570228, China.
J Plant Physiol. 2021 Nov;266:153529. doi: 10.1016/j.jplph.2021.153529. Epub 2021 Sep 22.
Potassium is a major cationic nutrient involved in numerous physiological processes in plants. The uptake of K is mediated by K channels and transporters, and the Shaker K channel gene family plays an essential role in K uptake and stress resistance in plants. However, little is known regarding this family in soybean. In this study, 14 members of the Shaker K channel gene family were identified in soybean and were classified into five groups. Protein domain analysis revealed that Shaker K channel gene members have an ion transport domain (ion trans), a cyclic nucleotide-binding domain, ankyrin repeat domains, and a dimerization domain in the potassium ion channel. Quantitative real-time polymerase chain reaction analysis indicated that the expression of eight genes (notably GmAKT1) in soybean leaves and roots was significantly increased in response to salt and drought stress. Furthermore, the overexpression of GmAKT1 in Arabidopsis enhanced root length, K concentration, and fresh/dry weight ratio compared with wild-type plants subjected to salt and drought stress; this suggests that GmAKT1 improves the tolerance of soybean to abiotic stress. Our results provide important insight into the characterization of Shaker K channel gene family members in soybean and highlight the function of GmAKT1 in soybean plants under salt and drought stress.
钾是一种主要的阳离子养分，参与植物的众多生理过程。钾的吸收由钾通道和转运体介导，而震荡器钾通道基因家族在植物钾吸收和抗逆性中起重要作用。然而，关于大豆中的这个家族我们了解甚少。在本研究中，在大豆中鉴定出了震荡器钾通道基因家族的14个成员，并将其分为五组。蛋白质结构域分析表明，震荡器钾通道基因成员在钾离子通道中具有离子转运结构域（ion trans）、环核苷酸结合结构域、锚蛋白重复结构域和二聚化结构域。定量实时聚合酶链反应分析表明，大豆叶片和根中八个基因（特别是GmAKT1）的表达在盐和干旱胁迫下显著增加。此外，与遭受盐和干旱胁迫的野生型植物相比，拟南芥中GmAKT1的过表达增加了根长、钾浓度以及鲜重/干重比；这表明GmAKT1提高了大豆对非生物胁迫的耐受性。我们的结果为大豆中震荡器钾通道基因家族成员的特征提供了重要见解，并突出了GmAKT1在盐和干旱胁迫下大豆植株中的功能。
