Immune Cells and Antibody Engineering Research Center of Guizhou Province/Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, 550025, Guizhou, China.
School of Biology and Engineering, Guizhou Medical University, Guiyang, 550025, Guizhou, China.
Biometals. 2021 Dec;34(6):1365-1379. doi: 10.1007/s10534-021-00347-4. Epub 2021 Oct 2.
Selenium is an essential trace element that can regulate the function of immnue cells via selenoproteins. However, the effects of selenium on human dendritic cell (DCs) remain unclear. Thus, selenoprotein levels in monocytes, immature DCs (imDCs) and mature DCs (mDCs) treated with or without NaSeO were evaluated using RT-PCR, and then the immune function of imDCs and mDCs was detected by flow cytometry, cell counting and the CCK8 assay. In addition, the effects of Se on cytokine and surface marker expression were investigated by RT-PCR. The results revealed different expression levels of selenoprotein in monocytes, imDCs and mDCs, and selenoproeins could be regulated by Se. Moreover, it was indicated that anti-phagocytic activity was improved by 0.1 µM Se, whereas it was suppressed by 0.2 µM Se in imDCs; The migration of imDCs and mDCs was improved by 0.1 µM Se, whereas their migration was inhibited by treatment with 0.05 or 0.2 µM Se; The mixed lymphocyte reaction of mDCs was improved by 0.1 µM Se, and it was inhibited by 0.05 and 0.2 µM Se. In addition, 0.1 µM Se improved the immune function of DCs through the regulation of CD80, CD86, IL12-p35 and IL12-p40. Wheres 0.05 and 0.2 µM Se impaired immune function of DCs by up-regulation of interleukin (IL-10) in imDCs and down-regulation of CD80, CD86, IL12-p35 and IL12-p40 in mDCs. In conclusion, 0.1 µM Se might improve the immune function of human DCs through selenoproteins.
硒是一种必需的微量元素，可以通过硒蛋白调节免疫细胞的功能。然而，硒对人类树突状细胞（DC）的影响尚不清楚。因此，使用 RT-PCR 评估了用或不用 NaSeO 处理的单核细胞、未成熟 DC（imDC）和成熟 DC（mDC）中的硒蛋白水平，然后通过流式细胞术、细胞计数和 CCK8 测定检测 imDC 和 mDC 的免疫功能。此外，通过 RT-PCR 研究了 Se 对细胞因子和表面标志物表达的影响。结果显示，单核细胞、imDC 和 mDC 中的硒蛋白表达水平不同，且硒蛋白可以被 Se 调节。此外，结果表明，0.1µM Se 可提高 imDC 的抗吞噬活性，而 0.2µM Se 则抑制其活性；0.1µM Se 可促进 imDC 和 mDC 的迁移，而 0.05µM 或 0.2µM Se 则抑制其迁移；0.1µM Se 可增强 mDC 的混合淋巴细胞反应，而 0.05µM 和 0.2µM Se 则抑制其反应。此外，0.1µM Se 通过调节 CD80、CD86、IL12-p35 和 IL12-p40 来改善 DC 的免疫功能。而 0.05µM 和 0.2µM Se 通过上调 imDC 中的白细胞介素（IL-10）和下调 mDC 中的 CD80、CD86、IL12-p35 和 IL12-p40 来损害 DC 的免疫功能。总之，0.1µM Se 可能通过硒蛋白改善人类 DC 的免疫功能。
