Sun Zhepeng, Liu Ci, Pan Tingru, Yao Haidong, Li Shu
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
Dev Comp Immunol. 2017 Dec;77:30-37. doi: 10.1016/j.dci.2017.07.018. Epub 2017 Jul 21.
Selenium (Se) promotes immune cell differentiation and improves immune response. Antigen-presenting cells such as dendritic cells (DCs) play an important role in immune system, however, the impact of Se on DCs is still unclear. In this study, we successfully induced and cultured chicken DCs from peripheral blood mononuclear cells by incubating mononuclear cells with 50 ng/mL recombinant chicken granulocyte-macrophage colony stimulating factor and 10 ng/mL recombinant chicken interleukin-4 for total 9 days. In + Se group, we added 10 mol/L sodium selenite from the first day of cell culture. The results showed that Se supplementation expedited and increased the expression of cell surface markers including CD11c, CD40, CD86, and MHC II. Principal component analysis showed that the expression of selenoproteins SelW, SelK, Dio3, GPX1, GPX2, SelN, SelS, SelH in chicken DCs was highly correlated, and SelW had highest correlation with the cell surface markers MHC II and CD11c. In conclusion, Se accelerates the differentiation and maturation of chicken DCs. Se regulates the differentiation and maturation of chicken DCs by selenoproteins. Selenoproteins has closely correlated to surface markers of chicken DCs.
硒（Se）可促进免疫细胞分化并改善免疫反应。抗原呈递细胞如树突状细胞（DCs）在免疫系统中发挥重要作用，然而，硒对树突状细胞的影响仍不清楚。在本研究中，我们通过将外周血单个核细胞与50 ng/mL重组鸡粒细胞-巨噬细胞集落刺激因子和10 ng/mL重组鸡白细胞介素-4孵育共9天，成功从外周血单个核细胞诱导培养出鸡树突状细胞。在+Se组中，从细胞培养的第一天起添加10 μmol/L亚硒酸钠。结果表明，补充硒可加速并增加包括CD11c、CD40、CD86和MHC II在内的细胞表面标志物的表达。主成分分析表明，鸡树突状细胞中硒蛋白SelW、SelK、Dio3、GPX1、GPX2、SelN、SelS、SelH的表达高度相关，且SelW与细胞表面标志物MHC II和CD11c的相关性最高。总之，硒可加速鸡树突状细胞的分化和成熟。硒通过硒蛋白调节鸡树突状细胞的分化和成熟。硒蛋白与鸡树突状细胞的表面标志物密切相关。
