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阿纳穆尔香蕉不同溶剂提取物的液相色谱-串联质谱表征、抗糖尿病、抗氧化和抗菌作用

LC-MS/MS characterization, antidiabetic, antioxidative, and antibacterial effects of different solvent extracts of Anamur banana ().

作者信息

Gedük Aysun Şener, Zengin Fatma

机构信息

Department of Food Engineering, Faculty of Engineering, University of Adana Alparslan Turkes Science and Technology, 01250 Saricam, Adana Turkey.

出版信息

Food Sci Biotechnol. 2021 Jul 28;30(9):1183-1193. doi: 10.1007/s10068-021-00953-5. eCollection 2021 Sep.

Abstract

The main objective of this study was to examine the phenolic compounds and the antibacterial, antioxidant, anti-α-glucosidase and anti-α-amylase activities of the different extracts (methanol, ethanol and hexane) of collected from the Anamur district in Turkey. LC-MS/MS was used to identify phenolic compounds. Quinic acid, acotinic acid, hesperidin and amentoflavone were identified in methanol extract. These phenolic compounds, excluding hesperidin, were also identified in the ethanol extract. Methanolic extract appeared the most active in all enzyme inhibition, antibacterial and antioxidative activity assays which is mainly due to its rich phenolic content. The methanol extract of banana showed the highest anti-α-glucosidase and anti-α-amylase activities with IC50 values of 5.45 ± 0.39 mg/mL, 9.70 ± 0.29 mg/mL, respectively. This study showed that methanol and ethanol extract, especially the methanol extract, have potential for use in the development of functional foods for reducing the diabetes and bacterial risks.

摘要

本研究的主要目的是检测从土耳其阿纳穆尔地区采集的[植物名称未给出]不同提取物(甲醇、乙醇和己烷)中的酚类化合物及其抗菌、抗氧化、抗α-葡萄糖苷酶和抗α-淀粉酶活性。采用液相色谱-串联质谱法(LC-MS/MS)鉴定酚类化合物。在甲醇提取物中鉴定出奎尼酸、刺桐酸、橙皮苷和穗花杉双黄酮。除橙皮苷外,这些酚类化合物也在乙醇提取物中被鉴定出来。在所有酶抑制、抗菌和抗氧化活性测定中,甲醇提取物表现出最高活性,这主要归因于其丰富的酚类含量。香蕉的甲醇提取物表现出最高的抗α-葡萄糖苷酶和抗α-淀粉酶活性,IC50值分别为5.45±0.39毫克/毫升、9.70±0.29毫克/毫升。本研究表明,甲醇和乙醇提取物,尤其是甲醇提取物,在开发用于降低糖尿病和细菌风险的功能性食品方面具有应用潜力。

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