Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University, Udgir, India.
Department of Medical Microbiology, Vilasrao Deshmukh Government Institute of Medical Research, Maharashtra University of Health Sciences, Latur, India.
Anim Biotechnol. 2023 Jun;34(3):746-755. doi: 10.1080/10495398.2021.1982725. Epub 2021 Oct 6.
A hospital-based cross-sectional study was conducted during 2018-2019 to decipher the prevalence of yeast mastitis. The results indicated a 19.68% prevalence of clinical mastitis in bovines. Among them, 5.51% of samples revealed yeasts constituting 1.09% overall prevalence. was recorded as a significant fungal agent involved in clinical bovine mastitis. We record the association of in clinical bovine mastitis. On proteomic and molecular confirmation, isolates were re-identified from phenotypically identified isolates associated with bovine mastitis. After conventional identification, the yeast isolates were re-identified by MALDI-TOF MS-based proteomic approaches. The D1/D2 domains of 26S-rRNA gene and 5.8S-internal transcribed spacer (ITS) rDNA regions based molecular phylogenetic analysis identified the isolates as . The isolates were resistant to fluconazole. This study reports the first systemic study of isolates recovered from bovine clinical mastitis, utilizing conventional, automated, proteomic, and genomic approaches followed by antifungal susceptibility. The findings suggest as a potent opportunistic emerging pathogen of veterinary and public health concern, need for accurate identification of fungal agents from mycotic mastitis, and use of validated antifungal susceptibility assay because of developing resistance to antimycotic agents. Our findings suggest judicious use of fluconazole and alternative antifungal agents may be considered in case of an outbreak.
本研究于 2018-2019 年开展了一项基于医院的横断面研究，旨在解析酵母性乳腺炎的流行情况。结果显示，牛的临床乳腺炎患病率为 19.68%。其中，5.51%的样本显示出酵母，总患病率为 1.09%。被记录为参与临床牛乳腺炎的重要真菌病原体。我们记录了在临床牛乳腺炎中与的关联。通过蛋白质组学和分子确认，从表型鉴定为与牛乳腺炎相关的分离株中重新鉴定出分离株。在常规鉴定后，通过 MALDI-TOF MS 基于蛋白质组学的方法重新鉴定酵母分离株。26S-rRNA 基因的 D1/D2 结构域和 5.8S 内部转录间隔区（ITS）rDNA 区域的分子系统发育分析将分离株鉴定为。分离株对氟康唑具有耐药性。本研究首次对从牛临床乳腺炎中分离出的进行了系统研究，利用常规、自动化、蛋白质组学和基因组学方法，以及抗真菌药敏试验。研究结果表明，作为一种具有潜在威胁的新兴兽医和公共卫生病原体，需要从真菌性乳腺炎中准确鉴定真菌病原体，并使用经过验证的抗真菌药敏试验，因为抗真菌药物的耐药性正在不断发展。本研究结果提示，在爆发时，应慎重使用氟康唑和其他替代抗真菌药物。
