Henthorn P S, Knoll B J, Raducha M, Rothblum K N, Slaughter C, Weiss M, Lafferty M A, Fischer T, Harris H
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5597-601. doi: 10.1073/pnas.83.15.5597.
Amino-terminal amino acid sequences (42 residues) were determined for the products of the three common alleles at the human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] gene locus. The sequences differ at position 3, which is proline in types 1 and 2 but is leucine in type 3. cDNA libraries were constructed in phage lambda gt11 and used to isolate clones covering the coding regions of types 1 and 3 cDNAs. Comparison of the deduced amino acid sequences of the types 1 and 3 proteins showed 7 differences out of 513 amino acids, each due to a single base substitution. cDNA sequence comparisons showed three silent substitutions in the coding regions and three base differences in the greater than 1 kilobase pairs of 3' untranslated sequences.
测定了人胎盘碱性磷酸酶[正磷酸单酯磷酸水解酶（最适碱性），EC 3.1.3.1]基因座上三个常见等位基因产物的氨基末端氨基酸序列（42个残基）。这些序列在第3位不同，1型和2型中为脯氨酸，而3型中为亮氨酸。构建了噬菌体λgt11 cDNA文库，并用于分离覆盖1型和3型cDNA编码区的克隆。1型和3型蛋白质推导的氨基酸序列比较显示，在513个氨基酸中有7个差异，每个差异都是由于单个碱基替换所致。cDNA序列比较显示，编码区有三个沉默替换，在大于1千碱基对的3'非翻译序列中有三个碱基差异。
