Abe E, Tanaka H, Ishimi Y, Miyaura C, Hayashi T, Nagasawa H, Tomida M, Yamaguchi Y, Hozumi M, Suda T
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5958-62. doi: 10.1073/pnas.83.16.5958.
Spleen cells treated with mitogens produce a potent bone-resorbing factor called osteoclast-activating factor (OAF). To examine the relationship between the bone-resorbing factor and other protein factors produced by spleen cells, the colony-stimulating factor (CSF), the differentiation-inducing factor (DIF), the macrophage fusion factor (MFF), and the macrophage growth factor (MGF) were purified from 2.68 liters of conditioned medium of mouse spleen cell cultures treated with concanavalin A. Purification was performed successively by DEAE-cellulose, Blue Sepharose, and Sephadex G-150 column chromatography and high-pressure liquid chromatography (HPLC). The DIF was successfully separated from CSF and MGF on HPLC. CSF coincided with MGF on HPLC, but MFF disappeared before application to HPLC. Only the DIF exhibited bone-resorbing activity, whereas CSF and MGF did not. The DIFs purified from L929 cells and Ehrlich ascites tumors similarly exhibited bone-resorbing activity. The DIFs purified from spleen cells and Ehrlich ascites tumor cells exhibited neither interleukin 1 (IL-1) activity nor tumor necrosis factor (TNF) activity, though the unfractionated conditioned medium from spleen cells did exhibit them. In the light of recent reports that IL-1 beta and TNF also stimulate bone resorption, the term OAF should refer to a generic activity rather than a single factor.
用丝裂原处理的脾细胞可产生一种名为破骨细胞激活因子（OAF）的强效骨吸收因子。为了研究这种骨吸收因子与脾细胞产生的其他蛋白质因子之间的关系，从2.68升用伴刀豆球蛋白A处理的小鼠脾细胞培养物的条件培养基中纯化了集落刺激因子（CSF）、分化诱导因子（DIF）、巨噬细胞融合因子（MFF）和巨噬细胞生长因子（MGF）。纯化过程依次通过DEAE-纤维素、蓝色琼脂糖和葡聚糖G-150柱色谱以及高压液相色谱（HPLC）进行。DIF在HPLC上成功地与CSF和MGF分离。CSF在HPLC上与MGF重合，但MFF在应用于HPLC之前就消失了。只有DIF表现出骨吸收活性，而CSF和MGF则没有。从L929细胞和艾氏腹水瘤中纯化的DIF同样表现出骨吸收活性。从脾细胞和艾氏腹水瘤细胞中纯化的DIF既不表现出白细胞介素1（IL-1）活性，也不表现出肿瘤坏死因子（TNF）活性，尽管脾细胞的未分级条件培养基确实表现出这些活性。鉴于最近有报道称IL-1β和TNF也刺激骨吸收，OAF这个术语应该指的是一种通用活性而非单一因子。
