Department of Clinical Laboratory, Zigong First People's Hospital, Zigong, China.
Department of Nuclear Medicine, Zigong First People's Hospital, Zigong, China.
J Physiol Anthropol. 2021 Oct 6;40(1):14. doi: 10.1186/s40101-021-00265-x.
To establish the reference interval of serum 25-hydroxyvitamin D (25(OH)D) in apparently healthy children in Zigong, China, using an indirect method to provide a basis for proper clinical diagnosis and treatment.
A total of 1851 apparently healthy children of the Children's Health Care Department in Zigong First People's Hospital between January 2016 and December 2020 were included in the study. The Kolmogorov-Smirnov test was used to analyze the data for normality, and the non-normally distributed data were transformed into approximately normal distribution by Blom's normal rank transformation, and the transformed data were excluded from outliers by the quartile spacing method, and the data were stratified and analyzed according to sex, age, and season. The data were stratified according to sex, age, and season, and the area between the 2.5% and 97.5% percentile points was used as the reference interval.
The serum 25(OH)D data were non-normally distributed. The data were normally distributed after Blom's normality rank transformation, and 92 cases of outliers were excluded from the transformed data according to the interquartile spacing method. The differences in serum 25(OH)D levels between sex were not statistically significant (P > 0.05), and there was no need to establish reference intervals based on sex. There was no statistically significant difference in serum 25-hydroxyvitamin D levels between winter and spring, and also no difference between summer and autumn (P > 0.05), and the levels were lower in winter-spring than in summer-autumn. Comparison between age groups showed that there was no statistically significant difference in serum 25(OH)D levels between the < 6 months group and the 6 ~ 11 months group, and between the 6 ~ 9 years group and the 10 ~ 14 years group (P > 0.05); serum 25(OH)D levels decreased with increasing age. There was an interactive effect of season and age group on 25(OH)D levels, and the corresponding reference intervals were established according to different seasons and age groups. In summer and autumn, the reference intervals of serum 25(OH)D for < 1 year, 1 ~ 2 years, 3 ~ 5 years, and 6 ~ 14 years were 39.86 ~ 151.43, 31.54 ~ 131.65, 22.05 ~ 103.75, and 15.36 ~ 85.53 ng/ml and 24.42 ~ 144.20, 31.54 ~ 131.65, 16.80 ~ 165.68, and 15.46 ~ 85.54 ng/ml in winter and spring, respectively.
Reference intervals for serum 25(OH)D in children of different seasons and ages in Zigong, China, were established to provide a reference for clinical disease diagnosis, treatment, and prognosis determination.
采用间接法建立中国自贡地区健康儿童血清 25-羟维生素 D（25（OH）D）的参考区间，为临床正确诊断和治疗提供依据。
选取 2016 年 1 月至 2020 年 12 月自贡市第一人民医院儿保科 1851 例健康儿童，采用 Kolmogorov-Smirnov 检验进行正态性分析，非正态分布数据采用 Blom 正态秩转换法转换为近似正态分布，采用四分位间距法剔除离群值，按性别、年龄和季节分层分析。按性别、年龄和季节分层，取第 2.5%和 97.5%百分位数之间的范围作为参考区间。
血清 25（OH）D 数据呈非正态分布。经 Blom 正态秩转换后数据呈正态分布，剔除转换数据中的 92 例离群值。血清 25（OH）D 水平在性别间差异无统计学意义（P>0.05），无需按性别建立参考区间。冬季和春季血清 25-羟维生素 D 水平差异无统计学意义（P>0.05），夏季和秋季也无差异（P>0.05），冬季和春季水平低于夏季和秋季。不同年龄组间比较显示，<6 个月组与 611 个月组、69 岁组与 1014 岁组血清 25（OH）D 水平差异无统计学意义（P>0.05）；血清 25（OH）D 水平随年龄增长而降低。季节和年龄组之间存在交互作用，根据不同季节和年龄组建立相应的参考区间。在夏季和秋季，<1 岁、12 岁、35 岁和 614 岁儿童血清 25（OH）D 的参考区间分别为 39.86151.43、31.54131.65、22.05103.75 和 15.3685.53ng/ml 及 24.42144.20、31.54131.65、16.80165.68 和 15.4685.54ng/ml；冬季和春季分别为 24.42144.20、31.54131.65、16.80165.68 和 15.4685.54ng/ml。
建立了中国自贡地区不同季节和年龄儿童血清 25（OH）D 的参考区间，为临床疾病诊断、治疗和预后判断提供参考。
