The Sackler Faculty of Medicine, Department of Epidemiology and Preventive Medicine, School of Public Health, Tel Aviv University, Tel Aviv, Israel.
Faculty of Life Sciences, The Shmunis School of Biomedicine and Cancer Research, Tel Aviv University, Tel Aviv, Israel.
Helicobacter. 2021 Dec;26(6):e12854. doi: 10.1111/hel.12854. Epub 2021 Oct 7.
Helicobacter pylori (H. pylori) infection is acquired during childhood and causes chronic gastritis that remains asymptomatic in most infected people. H. pylori alters the gastric microbiota and causes peptic ulcer disease. Evidence on the relationship between asymptomatic H. pylori infection and children's gut microbiota remains elusive.
We characterized the relationship between H. pylori infection and the intestinal microbiome of healthy children, adjusting for known inter-personal and environmental exposures.
This cross-sectional study included stool samples obtained from 163 Israeli Arab children aged 6-9 years from different socioeconomic strata. Sociodemographic information was collected through maternal interviews. H. pylori infection was determined using monoclonal antigen detection stool enzyme immunoassay. The gut microbiome was characterized by implementing 16S rRNA gene sequencing of the V4 region and a multivariate downstream analysis.
Overall, 57% of the participants were positive for H. pylori infection and it was significantly associated with low socioeconomic status. There was no significant association between H. pylori infection and bacterial richness of fecal microbiome. H. pylori infection was significantly associated with intestinal bacterial composition, including a strong association with Prevotella copri and Eubacterium biforme. Moreover, socioeconomic status was strongly associated with bacterial composition.
H. pylori infection in healthy children was significantly associated with altered intestinal microbiome structure. Socioeconomic determinants exhibit a strong effect, related to both H. pylori infection and intestinal diversity and composition in childhood. These findings are clinically important to the understanding of the role of H. pylori infection and other intestinal microbes in health and disease.
幽门螺杆菌（H. pylori）感染发生于儿童时期，导致慢性胃炎，多数感染者无症状。H. pylori 改变胃微生物群并引起消化性溃疡病。无症状 H. pylori 感染与儿童肠道微生物群之间的关系证据尚不清楚。
我们通过调整已知的人际和环境暴露因素，描述健康儿童中 H. pylori 感染与肠道微生物群的关系。
本横断面研究纳入了来自不同社会经济阶层的 163 名以色列阿拉伯儿童（6-9 岁）的粪便样本。通过母亲访谈收集社会人口学信息。使用单克隆抗原检测粪便酶免疫测定法确定 H. pylori 感染情况。通过 16S rRNA 基因 V4 区测序和多元下游分析来描述肠道微生物群。
总体而言，57%的参与者 H. pylori 感染阳性，且与低社会经济地位显著相关。H. pylori 感染与粪便微生物群的细菌丰富度无显著相关性。H. pylori 感染与肠道细菌组成显著相关，包括与普雷沃氏菌（Prevotella copri）和双歧杆菌（Eubacterium biforme）的强关联。此外，社会经济地位与细菌组成也有很强的关联。
健康儿童的 H. pylori 感染与肠道微生物群结构的改变显著相关。社会经济决定因素表现出很强的影响，与 H. pylori 感染以及儿童时期的肠道多样性和组成都有关。这些发现对理解 H. pylori 感染和其他肠道微生物在健康和疾病中的作用具有重要的临床意义。
