Bons N, Kerdelhué B, Assenmacher I
Cell Tissue Res. 1978 Mar 31;188(1):99-106. doi: 10.1007/BF00220517.
A fluorescent technique applying specific LHRH and vasotocin antisera was used for the immunocytochemical localization of the respective neurosecretory systems in the hypothalamus of gonadectomized, testosterone-treated and/or serotonin injected male domestic ducks. An immunoreactive (IR) LHRH-producing system, with perikarya located in the preoptic nucleus, could be traced through the ventral hypothalamus down to the external layer of the rostral and caudal ME, in close vicinity to the hypophysial portal system. An IR-vasotocin system originating in the paraventricular and supraoptic nuclei ran through the ventral hypothalamus, but terminated in (i) the external layer of the rostral ME, and (ii) in the posterior lobe of the hypophysis.
采用一种应用特异性促黄体生成素释放激素(LHRH)和血管催产素抗血清的荧光技术,对去势、经睾酮处理和/或注射血清素的雄性家鸭下丘脑内各自的神经分泌系统进行免疫细胞化学定位。一个产生免疫反应性(IR)促黄体生成素释放激素的系统,其核周体位于视前核,可经腹侧下丘脑追踪至吻侧和尾侧正中隆起(ME)的外层,紧邻垂体门脉系统。一个起源于室旁核和视上核的IR-血管催产素系统贯穿腹侧下丘脑,但终止于:(i)吻侧正中隆起的外层,以及(ii)垂体后叶。