State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Collaborative Innovation Centre of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing, 210037, China.
Chemosphere. 2022 Feb;288(Pt 2):132471. doi: 10.1016/j.chemosphere.2021.132471. Epub 2021 Oct 6.
Present study showed the successful application of the modified hydrothermal method for synthesizing the zinc oxide nanoparticles (ZnO-NPs) efficiently. Well as-synthesized ZnO-NPs are analyzed for various techniques viz., X-ray diffraction (XRD), SEM micrographs, EDAX/Mapping pattern, Raman Spectroscopy Pattern, UV, Photoluminescence (PL) and X-ray photoemission spectroscopy (XPS) analysis. All these measurements showed that ZnO-NPs are highly pure with no internal defects, and can be potentially used in the plant applications. Hence, we further determined the effect of these nanoparticles and melatonin for the modulation of the As tolerance in soybean plants by examining the various growth attributes and metabolic parameters. Our results demonstrated that As-stress inhibited growth (∼34%), photosynthesis-related parameters (∼18-28%) and induced ROS accumulation; however, all these attributes are substantially reversed by the ZnO-NPs and melatonin treatments. Moreover, the As stress induced malondialdehyde (MDA; 71%) and hydrogen peroxide (HO; 82%) are partially reversed by the ZnO-NPs and melatonin in the As-stressed plants. This might have resulted due to the ZnO-NPs and melatonin induced activities of the antioxidants plant defense. Overall, the ZnO-NPs and melatonin supplementation separately and in combination positively regulated the As tolerance in soybean; however, the effect of their combined application on the As tolerance was more profound relative to the individual application. These results suggested the synergetic effect of the ZnO-NPs and melatonin on the As tolerance in soybean. However, the in-depth mechanism underlying the defense crosstalk between the ZnO-NPs and melatonin needs to be further explored.
本研究成功应用改良水热法高效合成氧化锌纳米粒子（ZnO-NPs）。对合成的 ZnO-NPs 进行了各种技术分析，如 X 射线衍射（XRD）、SEM 显微照片、EDAX/图谱、拉曼光谱图、UV、光致发光（PL）和 X 射线光电子能谱（XPS）分析。所有这些测量结果表明，ZnO-NPs 高度纯净，没有内部缺陷，可潜在应用于植物。因此，我们进一步确定了这些纳米粒子和褪黑素对大豆植物砷耐受性的调节作用，通过检查各种生长属性和代谢参数。结果表明，砷胁迫抑制了生长（约 34%）、光合作用相关参数（约 18-28%）并诱导 ROS 积累；然而，所有这些特性都被 ZnO-NPs 和褪黑素处理显著逆转。此外，ZnO-NPs 和褪黑素部分逆转了砷胁迫下诱导的丙二醛（MDA；71%）和过氧化氢（HO；82%）的产生。这可能是由于 ZnO-NPs 和褪黑素诱导了植物防御系统中抗氧化剂的活性。总的来说，ZnO-NPs 和褪黑素的单独和联合补充都能正向调节大豆的砷耐受性；然而，与单独应用相比，它们联合应用对砷耐受性的影响更为显著。这些结果表明 ZnO-NPs 和褪黑素对大豆砷耐受性具有协同作用。然而，ZnO-NPs 和褪黑素之间防御交叉对话的深入机制需要进一步探索。
