Peng Li-Hua, Gu Ting-Wei, Xu Yang, Dad Haseeb Anwar, Liu Jian-Xiang, Lian Jia-Zhang, Huang Lu-Qi
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Biotechnol Adv. 2022 Jan-Feb;54:107845. doi: 10.1016/j.biotechadv.2021.107845. Epub 2021 Oct 7.
There are sharply rising demands for pharmaceutical proteins, however shortcomings associated with traditional protein production methods are obvious. Genetic engineering of plant cells has gained importance as a new strategy for protein production. But most current genetic manipulation techniques for plant components, such as gene gun bombardment and Agrobacterium mediated transformation are associated with irreversible tissue damage, species-range limitation, high risk of integrating foreign DNAs into the host genome, and complicated handling procedures. Thus, there is urgent expectation for innovative gene delivery strategies with higher efficiency, fewer side effect, and more practice convenience. Materials based nanovectors have established themselves as novel vehicles for gene delivery to plant cells due to their large specific surface areas, adjustable particle sizes, cationic surface potentials, and modifiability. In this review, multiple techniques employed for plant cell-based genetic engineering and the applications of nanovectors are reviewed. Moreover, different strategies associated with the fusion of nanotechnology and physical techniques are outlined, which immensely augment delivery efficiency and protein yields. Finally, approaches that may overcome the associated challenges of these strategies to optimize plant bioreactors for protein production are discussed.
对药用蛋白质的需求急剧上升，然而传统蛋白质生产方法的缺点显而易见。植物细胞基因工程作为一种新的蛋白质生产策略已变得越来越重要。但是，目前大多数用于植物成分的基因操作技术，如基因枪轰击和农杆菌介导的转化，都存在不可逆的组织损伤、物种范围限制、外源DNA整合到宿主基因组的高风险以及操作程序复杂等问题。因此，迫切需要创新的基因传递策略，以提高效率、减少副作用并增加操作便利性。基于材料的纳米载体因其大的比表面积、可调节的粒径、阳离子表面电位和可修饰性，已成为向植物细胞传递基因的新型载体。在这篇综述中，回顾了用于基于植物细胞的基因工程的多种技术以及纳米载体的应用。此外，还概述了与纳米技术和物理技术融合相关的不同策略，这些策略极大地提高了传递效率和蛋白质产量。最后，讨论了可能克服这些策略相关挑战以优化用于蛋白质生产的植物生物反应器的方法。
