Yin Huifang, Xue Guanhong, Dai Ailing, Wu Haichong
College of Life Sciences of Longyan University, Longyan, China.
Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, China.
Front Pharmacol. 2021 Sep 24;12:755768. doi: 10.3389/fphar.2021.755768. eCollection 2021.
Mastitis is a worldwide production disease in dairy cows, which mainly affects milk yield, causing huge economic losses to dairy farmers. Lentinan is a kind of polysaccharide extracted from , which has no toxicity and possesses various pharmacological activities including antibacterial and immunomodulatory effects. Therefore, the anti-inflammatory function of lentinan on LPS-stimulated mastitis was carried out, and the mechanism involved was explored. , lentinan greatly reduced LPS-stimulated pathological injury, myeloperoxidase (MPO) activity, and the proinflammatory factor production (TNF-α and IL-1β) in mice. Further study was performed to determine the activation of the Wnt/β-catenin pathway during LPS stimulation. These results suggested that LPS-induced activation of the Wnt/β-catenin pathway was suppressed by lentinan administration. , we observed that the mouse mammary epithelial cell (mMEC) viability was not affected by lentinan treatment. As expected, LPS increased the TNF-α and IL-1β protein secretion and the activation of the Wnt/β-catenin pathway that was inhibited by lentinan administration in a dose-dependent manner in mMECs. Conclusively, lentinan exerts the anti-inflammatory function in LPS-stimulated mastitis inhibiting the activation of the Wnt/β-catenin pathway. Thus, the results of our study also gave an insight that lentinan may serve as a potential treatment for mastitis.
乳腺炎是奶牛养殖业中一种全球性的生产性疾病，主要影响产奶量，给奶农造成巨大经济损失。香菇多糖是从[未提及具体来源]中提取的一种多糖，无毒且具有多种药理活性，包括抗菌和免疫调节作用。因此，研究了香菇多糖对脂多糖（LPS）刺激的乳腺炎的抗炎作用，并探讨了其作用机制。结果表明，香菇多糖能显著减轻LPS刺激引起的小鼠病理损伤、髓过氧化物酶（MPO）活性以及促炎因子（肿瘤坏死因子-α和白细胞介素-1β）的产生。进一步研究确定了LPS刺激过程中Wnt/β-连环蛋白信号通路的激活情况。这些结果表明，香菇多糖给药可抑制LPS诱导的Wnt/β-连环蛋白信号通路激活。此外，我们观察到香菇多糖处理对小鼠乳腺上皮细胞（mMEC）活力无影响。正如预期的那样，LPS增加了TNF-α和IL-1β蛋白分泌以及Wnt/β-连环蛋白信号通路的激活，而香菇多糖给药在mMECs中呈剂量依赖性抑制这种激活。总之，香菇多糖通过抑制Wnt/β-连环蛋白信号通路的激活在LPS刺激的乳腺炎中发挥抗炎作用。因此，我们的研究结果还表明香菇多糖可能是一种潜在的乳腺炎治疗药物。
