Zhao Xiaoya, Dong Bingqi, Friesen Marissa, Liu Shangxi, Zhu Changqing, Yang Chengbo
Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada.
School of Food Science, Nanjing Xiaozhuang University, Nanjing, China.
Front Physiol. 2021 Sep 24;12:715469. doi: 10.3389/fphys.2021.715469. eCollection 2021.
Capsaicin is a spicy, highly pungent, colorless, vanilloid compound found in chili peppers with anti-inflammatory, antioxidant, anti-cancer, and analgesic properties. However, the protective effects of capsaicin on the pig intestine during inflammation are yet to be explored. This study investigated the effects of capsaicin on the gut inflammatory response, intestinal epithelial integrity, and gene expression level of nutrient transporters in a model of lipopolysaccharide (LPS)-induced inflammation in non-differentiated intestinal porcine epithelial cell line-J2 (IPEC-J2). The results showed that the pre-treatment of cells with capsaicin (100 μM) significantly decreased the gene expression and secretion of proinflammatory cytokines induced by LPS through Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. In addition, pre-treatment of cells with capsaicin also increased both gene and protein abundance of tight junction proteins. Furthermore, pre-treatment cells with capsaicin significantly increased trans-epithelial electrical resistance (TEER) and decreased permeability of fluorescein isothiocyanate-dextran (FD4) from the apical side to the basolateral side compared with the control ( < 0.05). Additionally, pre-treatment of cells with capsaicin upregulated the mRNA abundance of nutrients transporters such as Na/glucose cotransporter 1 (SGLT1). These results suggested that capsaicin could attenuate LPS-induced inflammation response through TLR4/NF-κB pathway and improve barrier integrity and glucose absorption.
辣椒素是一种辛辣、高度刺鼻、无色的香草酸类化合物，存在于辣椒中，具有抗炎、抗氧化、抗癌和镇痛特性。然而，辣椒素在炎症期间对猪肠道的保护作用尚未得到探索。本研究在未分化的猪肠道上皮细胞系-J2（IPEC-J2）的脂多糖（LPS）诱导的炎症模型中，研究了辣椒素对肠道炎症反应、肠上皮完整性和营养转运蛋白基因表达水平的影响。结果表明，用辣椒素（100μM）预处理细胞可通过Toll样受体4（TLR4）/核因子κB（NF-κB）信号通路显著降低LPS诱导的促炎细胞因子的基因表达和分泌。此外，用辣椒素预处理细胞还增加了紧密连接蛋白的基因和蛋白质丰度。此外，与对照组相比，用辣椒素预处理细胞显著增加了跨上皮电阻（TEER），并降低了异硫氰酸荧光素-葡聚糖（FD4）从顶端侧到基底外侧的通透性（P<0.05）。此外，用辣椒素预处理细胞上调了营养转运蛋白如钠/葡萄糖共转运蛋白1（SGLT1）的mRNA丰度。这些结果表明，辣椒素可通过TLR4/NF-κB途径减轻LPS诱导的炎症反应，并改善屏障完整性和葡萄糖吸收。
