DNA vaccine containing gene induces significant immune responses against infection: An study.

OBJECTIVES

is one of the most prevalent human infectious agents that is directly involved in various upper digestive tract diseases. Although antibiotics-based therapy and proton pump inhibitors eradicate the bacteria mostly, their effectiveness has been declined recently due to emergence of antibiotic-resistant strains. Development of a DNA vaccine is a promising approach against bacterial pathogens. Genes encoding motility factors are promising immunogens to develop a DNA vaccine against infection due to critical role of these genes in bacterial attachment and colonization within the gastric lumen. The present study aimed to synthesize a DNA vaccine construct based on the gene , the predominant flagellin subunit in flagella.

MATERIALS AND METHODS

The coding sequence of was amplified through PCR and sub-cloned in the pBudCE4.1 vector. The recombinant vector was introduced into the human dermal fibroblast cells, and its potency to express the flaA protein was analyzed using SDS-PAGE. The recombinant construct was intramuscularly (IM) injected into the mice, and the profiles of cytokines and immunoglobulins were measured using ELISA.

RESULTS

It has been found that was successfully expressed in cells. Recombinant-vector also increased the serum levels of evaluated cytokines and immunoglobulins in mice.

CONCLUSION

These findings showed that the pBudCE4.1- construct was able to activate the immune responses. This study is the first step towards synthesis of recombinant-construct based on the gene. Immunization with such construct may inhibit the -associated infection; however, further experiments are urgent.