Institute of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong 250014, China.
Shandong Provincial Key Laboratory of Animal Resistant, School of Life Sciences, Shandong Normal University, Jinan, Shandong 250014, China.
Aging (Albany NY). 2021 Oct 11;13(19):23245-23261. doi: 10.18632/aging.203616.
Non-small cell lung cancer (NSCLC) is the most common type in lung cancer in the world, and it severely threatens the life of patients. Resveratrol has been reported to inhibit cancer. However, mechanisms of resveratrol inhibiting NSCLC were unclear. The aim of this study was to identify differentially expressed genes (DEGs) of NSCLC treated with resveratrol and reveal the potential targets of resveratrol in NSCLC. We obtained mRNA expression profiles of two datasets from the National Center for Biotechnology Information Gene Expression Omnibus (NCBI-GEO) and 271 DEGs were selected for further analysis. Data from STRING shown that 177 nodes and 342 edges were in the protein-protein interaction (PPI) network, and 10 hub genes (, , , , , , , , , and ) were identified by Cytoscape plus-in cytoHubba. Survival analysis revealed that NSCLC patients showing low expression of , , , , and were associated with worse overall survival (OS) ( < 0.05), and high expression of and was associated with worse OS for NSCLC patients ( < 0.05). Overall, we identified , , , and as potential candidate genes which were main effects of resveratrol on the treatment of NSCLC. , , , and are all clusters of differentiation (CD) antigens, might be the targets of resveratrol. The bioinformatic results suggested that the inhibitory effect of resveratrol on lung cancer may be related to the immune signaling pathway. Further studies are needed to validate these findings and to explore their functional mechanisms.
非小细胞肺癌（NSCLC）是世界上最常见的肺癌类型，严重威胁着患者的生命。白藜芦醇已被报道具有抑制癌症的作用。然而，白藜芦醇抑制 NSCLC 的机制尚不清楚。本研究旨在鉴定白藜芦醇处理的 NSCLC 的差异表达基因（DEGs），并揭示白藜芦醇在 NSCLC 中的潜在靶点。我们从国家生物技术信息中心基因表达综合数据库（NCBI-GEO）获得了两个数据集的 mRNA 表达谱，选择了 271 个差异表达基因进行进一步分析。STRING 数据显示，蛋白质-蛋白质相互作用（PPI）网络中有 177 个节点和 342 个边，Cytoscape 加 cytoHubba 鉴定出 10 个枢纽基因（、、、、、、、、和）。生存分析显示，NSCLC 患者中、、、、和的低表达与总生存期（OS）较差相关（<0.05），而和的高表达与 NSCLC 患者的 OS 较差相关（<0.05）。总之，我们鉴定出、、、和作为白藜芦醇治疗 NSCLC 的潜在候选基因，它们都是白藜芦醇的主要作用靶点。、、、和都是分化簇（CD）抗原，可能是白藜芦醇的靶点。这些生物信息学结果表明，白藜芦醇对肺癌的抑制作用可能与免疫信号通路有关。需要进一步的研究来验证这些发现并探讨它们的功能机制。
