Cummings J, Morrison J G, Willmott N
J Chromatogr. 1986 Sep 5;381(2):373-84. doi: 10.1016/s0378-4347(00)83603-8.
We describe the application of a high-speed spectrophotometric detector and high-performance liquid chromatography to the determination of anthracycline purity in extracted patient specimens and to the identification of chemical reduction products. Blood contained pure anthracyclines whilst in urine, tissue and tumour there was evidence of co-eluting endogenous peaks and complexation. Aerobic reduction yielded two main products: a C13 alcohol and a fully reduced, non-fluorescent, yellow hydroquinone. Anaerobic reduction in the presence of DNA yielded a 7-deoxyaglycone metabolite end product instead of the fully reduced hydroquinone. Eight other separate chromatographic species were identified, all of which showed unique absorbance characteristics, having a visible lambda max at 530 nm and being coloured purple/blue.
我们描述了高速分光光度检测器和高效液相色谱在测定提取的患者标本中蒽环类药物纯度以及鉴定化学还原产物方面的应用。血液中含有纯蒽环类药物，而在尿液、组织和肿瘤中，有共洗脱内源性峰和络合的证据。有氧还原产生两种主要产物：一种C13醇和一种完全还原的、无荧光的黄色对苯二酚。在DNA存在下的厌氧还原产生了一种7-脱氧糖苷配基代谢终产物，而不是完全还原的对苯二酚。还鉴定出了其他八个单独的色谱物种，所有这些物种都表现出独特的吸光度特征，在530 nm处有可见的最大吸收波长，并且呈紫色/蓝色。
