Pharmacology Department, National Research Centre, Dokki, Giza 12622, Egypt.
Chemistry of Natural and Micro and al Products Department, National Research Centre, Dokki, Giza 12622, Egypt.
Molecules. 2021 Sep 26;26(19):5833. doi: 10.3390/molecules26195833.
Different parts of (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world. The shoots essential oil (EO) has not yet been studied. Herein, the chemical profile of shoots EO (ABSEO) was created by GC-MS analysis. Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated. Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%). The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%). The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups. All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues. Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast. These findings advised that the use of ABSEO and its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents.
（布尼亚松）树的不同部位，如坚果、种子、树皮和嫩枝，在世界各地被广泛用于烹饪、茶饮和传统药物。嫩枝精油（ABSEO）尚未进行过研究。在此，通过 GC-MS 分析创建了 ABSEO 的化学成分图谱。此外，还评估了 ABSEO 及其纳米乳剂对卡拉胶诱导模型的体内口服和局部抗炎作用以及解热潜力。鉴定并分类了 43 种萜类化合物，分为单萜（42.94%）、倍半萜（31.66%）和二萜（23.74%）。ABSEO 的主要化合物为柏烯（20.81%）、α-蒎烯（16.21%）、D-柠檬烯（14.22%）、大根香叶烯 D（6.69%）、β-葎草烯（4.14%）和柠檬烯（4.12%）。ABSEO 和纳米乳剂在卡拉胶诱导的大鼠足肿胀模型中，无论是口服（50 和 100mg/kg）还是局部（5%大豆油）途径，均表现出显著的抗炎作用，与对照组和参比药物组相比。所有结果均表明，通过炎症细胞因子（IL-1β 和 IL8）、硝化（NO）和前列腺素 E2（PGE2）的减少，支持组织病理学研究和 MMP-9 和 NF-κβ 水平的免疫组化评估，从而显著减轻炎症。此外，ABSEO 和纳米乳剂（50 和 100mg/kg）的口服给药在大鼠中表现出解热活性，这是通过抑制肌内注射啤酒酵母引起的体温升高来证明的。这些发现表明，ABSEO 和纳米乳剂可用于治疗多种炎症和发热疾病，这可能归因于其有效成分。
