Analytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, University Innovation Incubator Building, SAINS@USM Campus, Lebuh Bukit Jambul, Bayan Lepas 11900, Penang, Malaysia.
Non-Destructive Biomedical and Pharmaceutical Research Centre, Smart Manufacturing Research Institute, Universiti Teknologi MARA Selangor, Puncak Alam 42300, Selangor, Malaysia.
Molecules. 2022 Dec 22;28(1):92. doi: 10.3390/molecules28010092.
Abnormal skin pigmentation commonly occurs during the wound healing process due to the overproduction of melanin. Chicken egg white (CEW) has long been used to improve skin health. Previous published works had found CEW proteins house bioactive peptides that inhibit tyrosinase, the key enzyme of melanogenesis. The current study aimed to evaluate the anti-pigmentation potential and mechanism of the CEW-derived peptide (GYSLGNWVCAAK) and hydrolysates (CEWH and CEWH), using a cell-based model. All of these peptide and hydrolysates inhibited intracellular tyrosinase activity and melanin level up to 45.39 ± 1.31 and 70.01 ± 1.00%, respectively. GYSLGNWVCAAK and CEWH reduced intracellular cAMP levels by 13.38 ± 3.65 and 14.55 ± 2.82%, respectively; however, CEWH did not affect cAMP level. Moreover, the hydrolysates downregulated the mRNA expression of melanogenesis-related genes, such as , , and , but GYSLGNWVCAAK only suppressed gene expression. Downregulation of the genes may lower the catalytic activities and/or affect the structural stability of TYR, TRP-1 and TRP-2; thus, impeding melanogenesis to cause an anti-pigmentation effect in the cell. Outcomes from the current study could serve as the starting point to understand the underlying complex, multifaceted melanogenesis regulatory mechanism at the cellular level.
异常的皮肤色素沉着在伤口愈合过程中很常见，这是由于黑色素的过度产生。鸡蛋蛋白（CEW）长期以来一直被用于改善皮肤健康。以前的研究已经发现 CEW 蛋白含有抑制酪氨酸酶的生物活性肽，酪氨酸酶是黑色素生成的关键酶。本研究旨在利用细胞模型评估 CEW 衍生肽（GYSLGNWVCAAK）和水解产物（CEWH 和 CEWH）的抗色素沉着潜力和机制。这些肽和水解产物都能抑制细胞内酪氨酸酶活性和黑色素水平，分别达到 45.39±1.31%和 70.01±1.00%。GYSLGNWVCAAK 和 CEWH 分别使细胞内 cAMP 水平降低了 13.38±3.65%和 14.55±2.82%；然而，CEWH 并不影响 cAMP 水平。此外，水解产物下调了黑色素生成相关基因的 mRNA 表达，如 、 、 和 ，但 GYSLGNWVCAAK 仅抑制了 基因的表达。这些基因的下调可能会降低 TYR、TRP-1 和 TRP-2 的催化活性和/或影响其结构稳定性；从而阻止黑色素生成，在细胞中产生抗色素沉着作用。本研究的结果可以为理解细胞水平上黑色素生成的复杂、多方面的调节机制提供起点。
