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从蓝色豆浆和豆腐中分离出的细菌色素产生的基因组序列草案及基因组分析

Draft Genome Sequences and Genomic Analysis for Pigment Production in Bacteria Isolated from Blue Discolored Soymilk and Tofu.

作者信息

De León Marina E, Wilson Harriet S, Jospin Guillaume, Eisen Jonathan A

机构信息

Department of Microbiology & Molecular Genetics, University of California, Davis, California 95616 USA.

UC Davis Genome Center, 451 Health Science Drive, Davis, CA 95616 USA.

出版信息

J Genomics. 2021 Sep 23;9:55-67. doi: 10.7150/jgen.65500. eCollection 2021.

Abstract

Cold-tolerant bacteria are known to contaminate and cause defects in refrigerated foods. Defects in food products can be observed as changes in appearance, texture, and/or flavor that detract from the product's intended look, feel, or taste. Two distinct organisms were cultured from blue pigmented soymilk and tofu that had been left opened and expired in a home refrigerator. The blue coloration was reproduced when isolates were cultured in fresh, sterile soymilk. These strains also produced a variety of colony color morphologies when cultured on different media types. We report two draft genome sequences of the potential causative agents of blue discoloration of soy foods, strains UCD_MED3 and UCD_MED7 as well as the 16S rRNA gene sequences of co-occurring strains isolated from the defective soy samples but that did not cause blue discoloration when cultured in fresh soymilk; strains UCD_MED2 and UCD_MED5.

摘要

已知耐冷细菌会污染冷藏食品并导致其出现缺陷。食品中的缺陷可表现为外观、质地和/或风味的变化,这些变化会降低产品预期的外观、口感或味道。从在家用冰箱中开封且过期的蓝色豆浆和豆腐中培养出了两种不同的微生物。当将分离菌株在新鲜无菌豆浆中培养时,会重现蓝色。这些菌株在不同类型的培养基上培养时还会产生多种菌落颜色形态。我们报告了大豆食品蓝色变色潜在致病因子的两个基因组草图序列,即菌株UCD_MED3和UCD_MED7,以及从有缺陷的大豆样品中分离出的同时存在的菌株的16S rRNA基因序列,但这些菌株在新鲜豆浆中培养时不会导致蓝色变色;这些菌株为UCD_MED2和UCD_MED5。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cf2/8490086/625cee64445e/jgenv09p0055g001.jpg

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