酿酒酵母中α因子生物合成的机制。
Mechanism of alpha factor biosynthesis in Saccharomyces cerevisiae.
作者信息
Scherer G, Haag G, Duntze W
出版信息
J Bacteriol. 1974 Aug;119(2):386-93. doi: 10.1128/jb.119.2.386-393.1974.
Abstract
The biosynthesis of alpha factor, a mating-type-specific regulatory oligopeptide which is secreted by Saccharomyces cerevisiae cells of alpha mating type, was studied. In batch cultures only small amounts of the peptide were synthesized during the exponential growth phase. During the stationary phase, alpha factor was produced at a constant rate and accumulated in the culture medium. Inhibition of translation in wild-type cells by cycloheximide, or in mutant strains under conditions which blocked protein or ribonucleic acid (RNA) synthesis completely inhibited the production of alpha factor. These results indicate that the factor is produced by ribosomal translation of a specific messenger RNA and not by an extraribosomal mechanism of peptide synthesis.
摘要
对α因子的生物合成进行了研究，α因子是一种交配型特异性调节寡肽，由α交配型的酿酒酵母细胞分泌。在分批培养中，指数生长期仅合成少量该肽。在稳定期，α因子以恒定速率产生并积累在培养基中。用环己酰亚胺抑制野生型细胞中的翻译，或在完全阻断蛋白质或核糖核酸（RNA）合成的条件下抑制突变菌株中的翻译，均完全抑制了α因子的产生。这些结果表明，该因子是由特定信使RNA的核糖体翻译产生的，而不是由肽合成的非核糖体机制产生的。
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