Abbas Mazhar, Sharif Sumaira, Baig Ibrahim Salam, Anjum Rimsha, Riaz Muhammad, Rafique Muhammad Kamran, Rahman Abdur, Nazish Nadia, Al-Mijalli Samiah H, Iqbal Munawar
Department of Biochemistry, University of Veterinary and Animal Sciences (Jhang-Campus), Jhang, Pakistan.
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Dose Response. 2021 Oct 10;19(4):15593258211044062. doi: 10.1177/15593258211044062. eCollection 2021 Oct-Dec.
The secondary metabolites in plants are the basis of defense and stress balance, which is an important aspect in plant growth. The UV-B treatment (a biotic stress) and salt stress on bitter melon ( L.) were studied, and the impact of pre-sowing seed treatment was evaluated on the basis of biochemical and enzymatic biomarkers, antioxidants, and wound-healing potential during early growth stages.
The UV-B treatment for 5 and 10 min and salt stress 250 mM and 500 mM treatments were applied, and 21-day seedling tissue were collected for total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant, chlorophyll contents, hydrogen peroxide, total soluble sugar, enzymes activities, and wound-healing potential studies.
The TPC, TFC, diphenyl picrylhydrazyl (DPPH), chlorophyll contents, and total soluble sugar were recorded higher at 5 min treatment with UV-B and salt stress at 250 mM concentration. Antioxidant enzymes activities were recorded higher for 10 min UV-B treatment and 500 mM salt treatment. Wound-healing potential was found significant at 5 min treatment with UV-B radiation, which was studied in rabbits. The LC-MS analysis revealed a variety of phenolic compounds in the seedlings.
The study concluded that treatments significantly affect the biological activities of bitter melon seeds at the seedling stage, and the seeds contain important phenolic compounds responsible for its antioxidant potential and enzymatic activities. Future studies could be focused on the later stages of growth, development, and yield characteristics subjected to salt stress along with UV-B radiation treatment.
植物中的次生代谢产物是防御和应激平衡的基础，这是植物生长的一个重要方面。研究了紫外线-B处理（一种生物胁迫）和盐胁迫对苦瓜的影响，并基于生化和酶生物标志物、抗氧化剂以及早期生长阶段的伤口愈合潜力，评估了播种前种子处理的影响。
采用5分钟和10分钟的紫外线-B处理以及250毫摩尔和500毫摩尔的盐胁迫处理，收集21天龄幼苗组织进行总酚含量（TPC）、总黄酮含量（TFC）、抗氧化剂、叶绿素含量、过氧化氢、总可溶性糖、酶活性和伤口愈合潜力研究。
在紫外线-B处理5分钟和250毫摩尔浓度的盐胁迫下，TPC、TFC、二苯基苦味酰基肼（DPPH）、叶绿素含量和总可溶性糖的记录值较高。在紫外线-B处理10分钟和500毫摩尔盐处理下，抗氧化酶活性的记录值较高。在对兔子进行的研究中发现，紫外线-B辐射处理5分钟时伤口愈合潜力显著。液相色谱-质谱分析揭示了幼苗中存在多种酚类化合物。
该研究得出结论，这些处理显著影响苦瓜种子在幼苗阶段的生物活性，并且种子含有负责其抗氧化潜力和酶活性的重要酚类化合物。未来的研究可以集中在盐胁迫与紫外线-B辐射处理下生长、发育和产量特征的后期阶段。
