Nishad Jay Hind, Singh Arti, Bharti Rajnish, Prajapati Priyanka, Sharma Vijay Kumar, Gupta Vijai Kumar, Kharwar Ravindra Nath
Mycopathology and Microbial Technology Laboratory, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, India.
Agricultural Research Organization, Rishon LeZion, Israel.
Front Microbiol. 2021 Sep 17;12:725463. doi: 10.3389/fmicb.2021.725463. eCollection 2021.
The endophytic fungus was isolated from the stem of (Roxb.) Willd., commonly known as Ashok plant in India and Sri Lanka. Since no reports are available regarding epigenetic modulations by BRD4770 in microbial entities, . was treated with different concentrations of BRD4770 for this purpose and evaluated for its antioxidant and antibacterial potential against five human pathogenic bacteria, , methicillin-resistant (MRSA), , and The crude extract obtained from cultures treated with 100 nM concentration of BRD4770 showed increased antioxidant activity and inhibition zone against and MRSA, compared to the non-treated control. The composition of the non-treated and treated crude extract was analyzed, and induced compounds were identified with the help of Gas chromatography-mass spectrometry (GC-MS) and LC-ESI-MS/MS. LC-ESI-MS/MS analysis showed that berberine (antibacterial)-, caffeine-, and theobromine (antioxidant)-like compounds were induced in the BRD4770-treated crude extract. The presence of particular absorbance at a wavelength of 346.5 nm for berberine, 259.4 nm for caffeine, and 278.4 nm for theobromine in the reverse-phase high-performance liquid chromatography (HPLC) analysis of both BRD4770-treated crude metabolites and standard solution of the above compounds strongly supported the increased antibacterial and antioxidant activities that may be due to inducing the alterations in bioactivities of the BRD4770-treated culture.
内生真菌是从（罗克斯伯）威尔德的茎中分离出来的，在印度和斯里兰卡通常被称为阿育王树。由于目前尚无关于BRD4770对微生物进行表观遗传调控的报道，因此为此用不同浓度的BRD4770处理该内生真菌，并评估其对五种人类致病细菌（即金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌）的抗氧化和抗菌潜力。与未处理的对照相比，用100 nM浓度的BRD4770处理的培养物获得的粗提物显示出对大肠杆菌和耐甲氧西林金黄色葡萄球菌的抗氧化活性增加和抑菌圈增大。分析了未处理和处理后的粗提物的成分，并借助气相色谱-质谱联用仪（GC-MS）和液相色谱-电喷雾电离串联质谱仪（LC-ESI-MS/MS）鉴定了诱导产生的化合物。LC-ESI-MS/MS分析表明，在BRD4770处理的粗提物中诱导产生了黄连素（抗菌）、咖啡因和可可碱（抗氧化）类化合物。对BRD4770处理的粗代谢产物和上述化合物标准溶液进行反相高效液相色谱（HPLC）分析时，黄连素在346.5 nm波长处、咖啡因在259.4 nm波长处以及可可碱在278.4 nm波长处出现特定吸光度，这有力地支持了抗菌和抗氧化活性的增加可能是由于BRD4770处理的培养物生物活性发生改变所致。
