Hu Rui, Bi Rui, Jiang Lianyong, Yang Xuhui, Zhong Yuan, Xie Xiao
Department of Cardiothoracic Surgery Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
J Cancer. 2021 Sep 3;12(21):6393-6400. doi: 10.7150/jca.57814. eCollection 2021.
This study aims to determine the expression pattern of long non-coding RNA (lncRNA) TUSC8 in esophageal cancer tissues and cell lines, to investigate its effects on esophageal cancer cell proliferation and migration, and to explore the mechanism of TUSC8-mediated esophageal cancer suppression via VEGFA downregulation. TUSC8 levels in esophageal cancer tissues and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The influence of TUSC8 on clinical features in esophageal cancer patients was analyzed. After intervening TUSC8 expression in esophageal cancer cells, the proliferative and migratory abilities were examined in OE19 and TE-1 cells through a series of function experiments. The interaction between TUSC8 and VEGFA was assessed by the bioinformatics prediction and dual-luciferase reporter assay. Finally, the co-regulation of TUSC8 and VEGFA on esophageal cancer cell functions was evaluated. TUSC8 was downregulated in esophageal cancer tissues compared with normal ones. Identically, decreased TUSC8 expression was detected in esophageal cancer cell lines compared with control cells. Low TUSC8 expression predicted poor prognosis in patients with esophageal cancer. Knockdown of TUSC8 promoted the proliferative and migratory abilities in OE19 cells, whereas overexpression of TUSC8 resulted in opposite results in TE-1 cells. VEGFA was confirmed to be a target gene of TUSC8. Overexpression of VEGFA could reverse the regulatory effects of TUSC8 on esophageal cancer cell proliferation and migration. LncRNA TUSC8 is downregulated in esophageal cancer tissues and cell lines. TUSC8 inhibits the proliferative and migratory abilities in esophageal cancer cells by negatively regulating VEGFA.
本研究旨在确定长链非编码RNA（lncRNA）TUSC8在食管癌组织和细胞系中的表达模式，探讨其对食管癌细胞增殖和迁移的影响，并探究TUSC8通过下调VEGFA介导食管癌抑制的机制。通过定量实时聚合酶链反应（qRT-PCR）检测食管癌组织和细胞系中TUSC8的水平。分析TUSC8对食管癌患者临床特征的影响。在食管癌细胞中干预TUSC8表达后，通过一系列功能实验检测OE19和TE-1细胞的增殖和迁移能力。通过生物信息学预测和双荧光素酶报告基因检测评估TUSC8与VEGFA之间的相互作用。最后，评估TUSC8和VEGFA对食管癌细胞功能的共同调节作用。与正常组织相比，食管癌组织中TUSC8表达下调。同样，与对照细胞相比，在食管癌细胞系中检测到TUSC8表达降低。TUSC8低表达预示食管癌患者预后不良。敲低TUSC8可促进OE19细胞的增殖和迁移能力，而TUSC8过表达在TE-1细胞中则产生相反的结果。证实VEGFA是TUSC8的靶基因。VEGFA过表达可逆转TUSC8对食管癌细胞增殖和迁移的调节作用。lncRNA TUSC8在食管癌组织和细胞系中表达下调。TUSC8通过负向调节VEGFA抑制食管癌细胞的增殖和迁移能力。
