School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, China.
Shenzhen Institute of Research and Innovation, The University of Hong Kong, Shenzhen, China.
Oxid Med Cell Longev. 2021 Oct 6;2021:5187569. doi: 10.1155/2021/5187569. eCollection 2021.
Breast cancer (BCa) is the leading cause of women's death worldwide; among them, triple-negative breast cancer (TNBC) is one of the most troublesome subtypes with easy recurrence and great aggressive properties. Dunn has been used in the clinic of Chinese society for hundreds of years. Shreds of evidence showed that Dunn has a favorable outcome in the management of cancer. However, the anti-TNBC efficacy of Dunn percolation extract (SSP) and its underlying mechanisms have not been fully elucidated. Hence, the present study is aimed at evaluating the anti-TNBC potential of SSP both in vitro and in vivo, through the cell viability, morphological analysis of MDA-MB-231, LDH release assay, ROS assay, and the tests of GSH aborted pyroptotic noninflammasome signaling pathway. Survival analysis using the KM Plotter and TNM plot database exhibited the inhibition of transcription levels of caspase-4 and 9 related to low relapse-free survival in patients with BCa. Based on the findings, SSP possesses anti-TNBC efficacy that relies on ROS-induced noncanonical inflammasome pyroptosis in cancer cells. In this study, our preclinical evidence is complementary to the preceding clinic of Chinese society; studies on the active principles of SPP remain underway in our laboratory.
乳腺癌（BCa）是全球女性死亡的主要原因；其中，三阴性乳腺癌（TNBC）是最棘手的亚型之一，容易复发且侵袭性极强。丹参与中国社会的临床应用已有数百年的历史。有大量证据表明，丹参与癌症的治疗有良好的效果。然而，丹参与渗滤提取物（SSP）的抗 TNBC 功效及其潜在机制尚未完全阐明。因此，本研究旨在通过细胞活力、MDA-MB-231 的形态分析、LDH 释放试验、ROS 测定以及 GSH 阻断焦亡非炎症小体信号通路的试验，在体外和体内评估 SSP 的抗 TNBC 潜力。使用 KM Plotter 和 TNM plot 数据库进行生存分析显示，转录水平抑制与 BCa 患者无复发生存率低相关的 caspase-4 和 9。基于这些发现，SSP 具有抗 TNBC 功效，依赖于 ROS 诱导的癌细胞中非经典炎症小体焦亡。在本研究中，我们的临床前证据补充了中国社会之前的临床研究；我们实验室仍在对 SPP 的活性成分进行研究。
