佛波酯可增强外源基因在淋巴细胞中的瞬时表达。
Transient expression of foreign genes in lymphoid cells is enhanced by phorbol ester.
作者信息
Holbrook N J, Luethy J D, Kin J
出版信息
Mol Cell Biol. 1987 Jul;7(7):2610-3. doi: 10.1128/mcb.7.7.2610-2613.1987.
Abstract
In lymphoid cells and an erythroleukemia cell line, phorbol myristate acetate significantly enhanced (up to 90-fold) expression of chloramphenicol acetyltransferase driven by four unrelated viral transcriptional units. The results suggest that phorbol myristate acetate acts via a general mechanism to enhance gene expression in these cells and provides a convenient means for increasing transient expression of foreign DNA.
摘要
在淋巴细胞和一种红白血病细胞系中，佛波酯显著增强（高达90倍）了由四个不相关病毒转录单位驱动的氯霉素乙酰转移酶的表达。结果表明，佛波酯通过一种通用机制来增强这些细胞中的基因表达，并为增加外源DNA的瞬时表达提供了一种便捷方法。
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