Jiang Mengmeng, Xiao Yanyu, E Weigao, Ma Lifeng, Wang Jingjing, Chen Haide, Gao Ce, Liao Yuan, Guo Qile, Peng Jinrong, Han Xiaoping, Guo Guoji
Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Liangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, China.
Front Cell Dev Biol. 2021 Oct 1;9:743421. doi: 10.3389/fcell.2021.743421. eCollection 2021.
Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single cells covering major zebrafish cell types and constructed a systematic zebrafish cell landscape. We revealed single-cell compositions for 18 zebrafish tissue types covering both embryo and adult stages. Single-cell mapping of caudal fin regeneration revealed a unique characteristic of blastema population and key genetic regulation involved in zebrafish tissue repair. Overall, our single-cell datasets demonstrate the utility of zebrafish cell landscape resources in various fields of biological research.
斑马鱼已被发现是生物学和再生研究中的一种重要模式生物。然而，斑马鱼组织再生过程中的细胞组成和分子动态仍未得到充分了解。在这里，我们利用微孔测序技术分析了超过25万个覆盖斑马鱼主要细胞类型的单细胞，并构建了一个系统的斑马鱼细胞图谱。我们揭示了涵盖胚胎和成年阶段的18种斑马鱼组织类型的单细胞组成。尾鳍再生的单细胞图谱揭示了芽基群体的独特特征以及斑马鱼组织修复中涉及的关键基因调控。总体而言，我们的单细胞数据集证明了斑马鱼细胞图谱资源在生物学研究各个领域的实用性。
