Silva Draulio C, Freitas Ana Lucia P, Barros Francisco Clark N, Lins Kézia O A L, Alves Ana Paula N N, Alencar Nylane M N, de Figueiredo Ingrid S T, Pessoa Cláudia, de Moraes Manoel O, Costa-Lotufo Letícia V, Feitosa Judith P A, Maciel Jeanny S, de Paula Regina C M
Departamento de Bioquímica, Universidade Federal do Ceará, Ceará, Brazil.
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Ceará, Brazil.
Carbohydr Polym. 2012 Jan 4;87(1):139-145. doi: 10.1016/j.carbpol.2011.07.029. Epub 2011 Jul 27.
A hot water-extracted polysaccharide fraction (PFCM) of Passiflora edulis was characterized by microanalysis, infrared spectroscopy, NMR and high performance size-exclusion chromatography. The major component in PFCM is (1→4) linked galacturonic acid (esterified and unesterified). Neutral sugars such as arabinose, glucose, rhamnose, mannose, and fucose were also present. Traces of xylose and ribose were detected. The PFCM sample had a similar molar mass to that of pectin extracted from P. edulis under acidic conditions. Sarcoma 180 tumors treated with PFCM showed a growth inhibition ratio ranging from 40.59% to 48.73% depending on the dosage and type of PFCM administration (oral or intraperitoneal). Toxicological analysis shows that PFCM increases the cell types involved in primary defense mechanisms and no significant changes in the biochemical parameters and organs (e.g., kidney and liver) were observed. However, the use of PFCM treatment increased the spleen weight when compared with the use of 5-fluorouracil.
对西番莲热水提取的多糖组分（PFCM）进行了微量分析、红外光谱、核磁共振和高效尺寸排阻色谱表征。PFCM中的主要成分是（1→4）连接的半乳糖醛酸（酯化和未酯化的）。还存在阿拉伯糖、葡萄糖、鼠李糖、甘露糖和岩藻糖等中性糖。检测到微量的木糖和核糖。PFCM样品的摩尔质量与在酸性条件下从西番莲中提取的果胶相似。用PFCM处理的肉瘤180肿瘤根据PFCM给药的剂量和类型（口服或腹腔注射）显示出40.59%至48.73%的生长抑制率。毒理学分析表明，PFCM增加了参与主要防御机制的细胞类型，并且未观察到生化参数和器官（如肾脏和肝脏）有显著变化。然而，与使用5-氟尿嘧啶相比，使用PFCM治疗增加了脾脏重量。
