Smiderle F R, Olsen L M, Ruthes A C, Czelusniak P A, Santana-Filho A P, Sassaki G L, Gorin P A J, Iacomini M
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba, PR, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba, PR, Brazil.
Carbohydr Polym. 2012 Jan 4;87(1):368-376. doi: 10.1016/j.carbpol.2011.07.063. Epub 2011 Aug 5.
For many years mushrooms have been consumed and appreciated by their nutritional value, and medicinal properties. The traditional mushroom cultivation takes too long and the macrofungi biotechnology has not been explored in its full potential yet. The goal of this work was to observe if different carbon sources could improve the yield and diversify fungi nutrient composition in submerged culture. Pleurotus pulmonarius mycelia and exopolysacharide productions were evaluated using glucose, galactose, xylose and arabinose. The mycelia yield varied depending on the culture medium, and galactose showed to be the best carbon source to produce EPS. Samples that showed the highest protein contents were grown with xylose (19.44%) and arabinose (26.05%). Furthermore, the biomass cultivated with these carbohydrates and with galactose showed five essential amino acids. All cultured biomass showed low lipid contents (∼1%), being composed mainly of unsaturated fatty acids. All EPS fractions showed as main structures glucans and mannogalactans.
多年来，蘑菇因其营养价值和药用特性而被食用和重视。传统的蘑菇栽培耗时过长，大型真菌生物技术的潜力尚未得到充分挖掘。这项工作的目标是观察不同碳源是否能提高深层培养中真菌的产量并使其营养成分多样化。使用葡萄糖、半乳糖、木糖和阿拉伯糖评估了肺形侧耳菌丝体和胞外多糖的产量。菌丝体产量因培养基而异，半乳糖被证明是产生胞外多糖的最佳碳源。蛋白质含量最高的样品是用木糖（19.44%）和阿拉伯糖（26.05%）培养的。此外，用这些碳水化合物和半乳糖培养的生物质含有五种必需氨基酸。所有培养的生物质脂质含量都很低（约1%），主要由不饱和脂肪酸组成。所有胞外多糖组分的主要结构都是葡聚糖和甘露半乳聚糖。
