Srivastava Subodh K, Knight Leandra M, Nakhla Mark, Abad Z Gloria
United States Department of Agriculture-Animal and Plant Health Inspection Service Plant Protection and Quarantine, S&T, Pathogen Confirmatory Diagnostics Laboratory, Beltsville, MD 20705.
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695.
Phytopathology. 2022 Apr;112(4):953-955. doi: 10.1094/PHYTO-04-21-0167-A. Epub 2022 Mar 17.
is one of the most important genera of plant pathogens, with many members causing high economic losses worldwide. To build robust molecular identification systems, it is very important to have information from well-authenticated specimens and, in preference, the ex-type specimens. The reference genomes of well-authenticated specimens form a critical foundation for genetics, biological research, and diagnostic applications. In this study, we describe four draft genome resources for the ex-type of BL34 (P0716 WPC) (118 contigs for 50 Mb), and well-authenticated specimens of BL57G (P10330 WPC) (591 contigs for 75 Mb), BL41G (P3822 WPC) (404 contigs for 84 Mb), and BL162 (P6303 WPC) (3,984 contigs for 108 Mb) generated with MinION long-read high-throughput sequencing technology (Oxford Nanopore Technologies). Using the quality reads, we assembled high-coverage genomes of with 291× coverage and 16,662 annotated genes; with 205× coverage and 29,271 annotated genes; with 76× coverage and 23,331 annotated genes, and with 42× coverage and 21,762 annotated genes. With the availability of genome sequences and their annotations, we predict that these draft genomes will be accommodating for various basic and applied research, including diagnostics to protect global agriculture.
是植物病原体中最重要的属之一，其许多成员在全球范围内造成了巨大的经济损失。为了建立强大的分子鉴定系统，拥有来自经过充分验证的标本，最好是模式标本的信息非常重要。经过充分验证的标本的参考基因组为遗传学、生物学研究和诊断应用奠定了关键基础。在本研究中，我们描述了模式菌株BL34（P0716 WPC）（50 Mb，118个重叠群）、经过充分验证的菌株BL57G（P10330 WPC）（75 Mb，591个重叠群）、BL41G（P3822 WPC）（84 Mb，404个重叠群）和BL162（P6303 WPC）（108 Mb，3984个重叠群）的四个基因组草图资源，这些资源是使用MinION长读长高通量测序技术（牛津纳米孔技术公司）生成的。利用高质量的读段，我们组装了高覆盖度的基因组，其中基因组覆盖度为291倍，注释基因有16,662个；基因组覆盖度为205倍，注释基因有29,271个；基因组覆盖度为76倍，注释基因有23,331个，基因组覆盖度为42倍，注释基因有21,762个。随着基因组序列及其注释的可得性，我们预测这些基因组草图将适用于各种基础研究和应用研究，包括用于保护全球农业的诊断研究。
