Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Dokki 12618, Giza, Egypt.
J Agric Food Chem. 2021 Nov 3;69(43):12785-12793. doi: 10.1021/acs.jafc.1c03825. Epub 2021 Oct 20.
The cultivated tomato suffered a severe attack by the whitefly (Gennadius), causing damage to leaves by feeding as well as transmitting the tomato yellow leaf curl virus (TYLCV), while the wild tomato is considerably less appealing to this insect species. It is reported that shows innate avoidance to -curcumene, which is produced naturally by . However, the mechanisms involved in the avoidance behavior of in response to this chiral compound are still unclear yet. In this study, the functional and binding characterization of odorant-binding protein 1 of (BtOBP1) were examined and against -curcumene. The obtained results showed that BtOBP1 exhibits specific binding activity to -curcumene, which acts as repellents to . By using a fluorescence-based binding assay, the difference of binding-affinity for -curcumene between wild type BtOBP1 and the mutant BtOBP1 to -curcumene was performed, which resulted in a single amino acid mutation (ASN108 > SER); moreover, BtOBP1-N108 displays significantly decreased binding affinities to -curcumene. Most interestingly, a knock-down experiment with the BtOBP1 showed that the whitefly responses to -curcumene are impaired. This study illustrated that BtOBP1 is a crucial protein involved in the perception and discrimination of -curcumene. Our findings may provide an excellent chance of finding a suitable antagonist of eco-friendly features that can block the perception of chemosensory signals in insects, preventing behaviors like food-finding.
栽培番茄遭受粉虱（Gennadius）的严重侵袭，其取食和传播番茄黄曲叶病毒（TYLCV）会损害叶片，而野生番茄对这种昆虫的吸引力要小得多。据报道，野生番茄先天避免接触β-姜黄烯，β-姜黄烯是由 产生的。然而， 对这种手性化合物的回避行为所涉及的机制尚不清楚。在这项研究中，研究人员检测了 （BtOBP1）的功能和结合特性，并研究了其对 β-姜黄烯的结合活性。研究结果表明，BtOBP1 对 β-姜黄烯具有特异性结合活性，β-姜黄烯对 具有驱避作用。通过荧光结合分析，对野生型 BtOBP1 和突变型 BtOBP1 对 β-姜黄烯的结合亲和力进行了比较，结果导致单个氨基酸突变（ASN108> SER）；此外，BtOBP1-N108 对 β-姜黄烯的结合亲和力显著降低。最有趣的是，BtOBP1 的敲低实验表明，粉虱对 β-姜黄烯的反应受到损害。这项研究表明，BtOBP1 是一种参与感知和区分 β-姜黄烯的关键蛋白。我们的研究结果可能为寻找具有生态友好特性的合适拮抗剂提供了极好的机会，这种拮抗剂可以阻断昆虫对化学感觉信号的感知，防止觅食等行为。
