Department of Bioengineering, 14716University of Missouri, Columbia, MO, USA.
J Biomater Appl. 2022 Feb;36(7):1289-1300. doi: 10.1177/08853282211051586. Epub 2021 Oct 21.
Post-traumatic osteoarthritis (PTOA) is a progressive articular degenerative disease that degrades articular cartilage and stimulates apoptosis in chondrocyte cells. An injectable decellularized, extracellular matrix (ECM) scaffold, that might be able to combat the effects of PTOA, was developed where the ECM was conjugated with 20 nm gold nanoparticles (AuNP) and supplemented with curcumin and hyaluronic acid (HA). Porcine diaphragm ECM was decellularized and homogenized; AuNPs were conjugated using chemical crosslinking followed by mixing with curcumin and/or HA. Injection force testing and scanning electron microscopy with energy-dispersive X-ray spectroscopy were utilized to characterize the ECM scaffolds. testing with L929 murine fibroblasts, equine synovial fibroblasts, and Human Chondrocytes were used to determine biocompatibility, reactive oxygen species (ROS) reduction, and chondroprotective ability. The results demonstrated that conjugation of 20 nm AuNPs to the ECM was successful without significantly altering the physical properties as noted in the low injection force. work provided evidence of biocompatibility with a propensity to reduce intracellular ROS and an ability to mitigate apoptosis of chondrocyte cells stimulated with IL-1β, a known apoptosis inducing cytokine. It was concluded that an injectable AuNP-ECM may have the ability to mitigate inflammation and apoptosis.
创伤后骨关节炎（PTOA）是一种进行性关节退行性疾病，会降解关节软骨并刺激软骨细胞凋亡。一种可注射的去细胞化细胞外基质（ECM）支架被开发出来，以对抗 PTOA 的影响，其中 ECM 与 20nm 金纳米颗粒（AuNP）结合，并补充了姜黄素和透明质酸（HA）。猪横膈膜 ECM 被去细胞化并均质化；使用化学交联将 AuNPs 连接，然后与姜黄素和/或 HA 混合。利用注射力测试和带有能量色散 X 射线光谱的扫描电子显微镜来表征 ECM 支架。使用 L929 鼠成纤维细胞、马滑膜成纤维细胞和人软骨细胞进行测试，以确定生物相容性、活性氧（ROS）减少和软骨保护能力。结果表明，20nm AuNP 与 ECM 的结合成功，而不会显著改变如低注射力所示的物理性质。这项工作提供了生物相容性的证据，具有降低细胞内 ROS 的趋势，并具有减轻由已知的凋亡诱导细胞因子 IL-1β刺激的软骨细胞凋亡的能力。结论是，可注射的 AuNP-ECM 可能具有减轻炎症和凋亡的能力。
