1 Department of Bioengineering, College of Engineering, University of Missouri , Columbia, Missouri.
2 Department of Veterinary Pathology, University of Missouri , Columbia, Missouri.
Tissue Eng Part A. 2018 Jul;24(13-14):1091-1098. doi: 10.1089/ten.TEA.2017.0385. Epub 2018 Mar 20.
Collagen soft tissue fillers suffer from fast reabsorption, which minimizes their use as a tissue-engineered construct. Extensive cross-linking can be utilized to extend longevity, but changes in microstructure and biomechanics can have deleterious effects. To enhance longevity while still achieving a natural microstructure, gold nanoparticles (AuNPs) were conjugated to fibrilized collagen and homogenized into an injectable form for use as a soft tissue filler. A long-term animal study in Yucatan swine was conducted to assess biocompatibility and longevity. Two formulations of the AuNP-collagen were compared to porcine cross-linked collagen and commercially available hyaluronic acid (HA). The results of the study demonstrated that the AuNPs may provide enhanced longevity over 6 months compared to HA and cross-linked collagen. Irritation scores indicated that the AuNP-collagen construct (AuNP-CC) demonstrated low irritation compared to the cross-linked collagen and HA while histology scores demonstrated good biocompatibility. Overall, it may be possible to utilize AuNPs to stabilize and increase the longevity of CC while still achieving biocompatibility.
胶原软组织填充剂存在快速吸收的问题，这极大地限制了其作为组织工程构建物的应用。广泛的交联可以延长其寿命，但微结构和生物力学的改变可能会产生有害影响。为了在保持自然微结构的同时延长寿命，将金纳米颗粒（AuNPs）与纤维状胶原结合，并均匀混合成可注射形式，用作软组织填充剂。对尤卡坦猪进行了一项长期动物研究，以评估其生物相容性和寿命。将两种 AuNP-胶原配方与猪交联胶原和市售透明质酸（HA）进行了比较。研究结果表明，与 HA 和交联胶原相比，AuNPs 可能提供超过 6 个月的增强寿命。刺激评分表明，与交联胶原和 HA 相比，AuNP-胶原构建物（AuNP-CC）的刺激程度较低，而组织学评分则表明其具有良好的生物相容性。总的来说，可能可以利用 AuNPs 来稳定和延长 CC 的寿命，同时仍保持其生物相容性。
