Vázquez Xenia, Fernández Javier, Bances Margarita, Lumbreras Pilar, Alkorta Miriam, Hernáez Silvia, Prieto Elizabeth, de la Iglesia Pedro, de Toro María, Rodicio M Rosario, Rodicio Rosaura
Área de Microbiología, Departamento de Biología Funcional, Universidad de Oviedo (UO), Oviedo, Spain.
Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Front Microbiol. 2021 Oct 5;12:720449. doi: 10.3389/fmicb.2021.720449. eCollection 2021.
serovar Kentucky (. Kentucky) with sequence type (ST) 198 and highly resistant to ciprofloxacin (ST198-Cip ) has emerged as a global MDR clone, posing a threat to public health. In the present study, whole genome sequencing (WGS) was applied to characterize all Cip Kentucky detected in five Spanish hospitals during 2009-2018. All Cip isolates ( = 13) were ST198 and carried point mutations in the quinolone resistance-determining regions (QRDRs) of both (resulting in Ser83Phe and Asp87Gly, Asp87Asn, or Asp87Tyr substitutions in GyrA) and (with Thr57Ser and Ser80Ile substitutions in ParC). Resistances to other antibiotics (ampicillin, chloramphenicol, gentamicin, streptomycin, sulfonamides, and tetracycline), mediated by the , , , , , and (A) genes, and arranged in different combinations, were also observed. Analysis of the genetic environment of the latter resistance genes revealed the presence of multiple variants of SGI1 ( genomic island 1)-K and SGI1-P, where all these resistance genes except were placed. IS elements, found at multiple locations within the SGI1 variants, have probably played a crucial role in their generation. Despite the wide diversity of SGI1-K- and SGI1-P-like structures, phylogenetic analysis revealed a close relationship between isolates from different hospitals, which were separated by a minimum of two and a maximum of 160 single nucleotide polymorphisms. Considering that isolates resistant to fluoroquinolones belong to the high priority list of antibiotic-resistant bacteria compiled by the World Health Organization, continuous surveillance of the . Kentucky ST198-CIP clone is required.
具有序列型（ST）198且对环丙沙星高度耐药（ST198 - Cip ）的肯塔基血清型（. Kentucky）已成为一种全球多重耐药克隆，对公共卫生构成威胁。在本研究中，应用全基因组测序（WGS）来表征2009 - 2018年期间在五家西班牙医院检测到的所有Cip 肯塔基菌株。所有Cip 分离株（n = 13）均为ST198，且在gyrA（导致GyrA中Ser83Phe和Asp87Gly、Asp87Asn或Asp87Tyr替换）和parC（ParC中Thr57Ser和Ser80Ile替换）的喹诺酮耐药决定区（QRDRs）携带点突变。还观察到由blaTEM、blaSHV、blaCTX - M、aadA、sul1和tet(A)基因介导的对其他抗生素（氨苄西林、氯霉素、庆大霉素、链霉素、磺胺类药物和四环素）的耐药性，且这些基因以不同组合排列。对后述耐药基因的遗传环境分析揭示了SGI1（基因组岛1）-K和SGI1 - P的多种变体的存在，除blaTEM外所有这些耐药基因都位于其中。在SGI1变体的多个位置发现的插入序列元件（IS元件）可能在它们的产生中起了关键作用。尽管SGI1 - K和SGI1 - P样结构具有广泛多样性，但系统发育分析显示来自不同医院的分离株之间存在密切关系，它们之间最少相隔两个单核苷酸多态性，最多相隔160个单核苷酸多态性。鉴于对氟喹诺酮耐药的分离株属于世界卫生组织编制的抗生素耐药细菌高优先级清单，需要对. Kentucky ST198 - CIP 克隆进行持续监测。
