MicroNano System Research Center, College of Information & Computer Engineering, Key Laboratory of Advanced Transducers and Intelligent Control System of Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, China.
Department of Vascular Surgery, Chinese PLA General Hospital, Beijing 100853, China.
ACS Sens. 2021 Nov 26;6(11):3933-3939. doi: 10.1021/acssensors.1c00802. Epub 2021 Oct 22.
Lysozymes in human urine have crucial clinical significance as an indicator of renal tubular and glomerular diseases. Most lysozyme detection methods rely on the enzyme-linked immunosorbent assay (ELISA), which is usually a tedious procedure. Meanwhile, aptamer sensors and fluorescence-based techniques for lysozyme detection have emerged in recent studies. However, these methods are time-consuming and highly complex in operation, and some even require exorbitant reagents and instruments, which restricts real-time clinical monitoring as diagnostic approaches. Therefore, a rapid and low-cost lysozyme detection method with facile preparation is still in demand for modern precision medicine. Herein, we propose a magnetoelastic (ME) immunosensor for lysozyme detection by detecting changes in resonance frequency under a magnetostrictive effect. The detection system is composed of a magnetoelastic chip with an immobilized lysozyme antibody, a solenoid coil, and a vector network analyzer. Since the ME sensor is ultrasensitive to mass change, the frequency offset caused by mass change can be utilized to detect the content of lysozyme. The immunosensor is evaluated to possess superior sensitivity of 138 Hz/μg mL in terms of the resonance frequency shift (RFS). In addition, our sensor displays an outstanding performance in specificity experiments and shows a relatively lower detection limit (1.26 ng/mL) than other conventional lysozyme detection methods (such as ELISA, chemiluminescence assay, fluorescence, and aptamer biosensors).
人尿中的溶菌酶作为肾小管和肾小球疾病的指标具有重要的临床意义。大多数溶菌酶检测方法依赖于酶联免疫吸附测定（ELISA），这通常是一个繁琐的过程。同时，适体传感器和基于荧光的溶菌酶检测技术在最近的研究中也已经出现。然而，这些方法操作繁琐，高度复杂，有些甚至需要昂贵的试剂和仪器，这限制了作为诊断方法的实时临床监测。因此，一种快速、低成本、易于制备的溶菌酶检测方法仍然是现代精准医学的需求。在这里，我们提出了一种基于磁弹性（ME）的免疫传感器，通过检测磁致伸缩效应下共振频率的变化来检测溶菌酶。检测系统由一个带有固定化溶菌酶抗体的磁弹性芯片、一个螺线管线圈和一个矢量网络分析仪组成。由于 ME 传感器对质量变化非常敏感，因此可以利用质量变化引起的频率偏移来检测溶菌酶的含量。该免疫传感器在共振频率偏移（RFS）方面表现出了优越的灵敏度，为 138 Hz/μg mL。此外，我们的传感器在特异性实验中表现出了出色的性能，并且与其他常规溶菌酶检测方法（如 ELISA、化学发光测定、荧光和适体生物传感器）相比，具有相对较低的检测限（1.26 ng/mL）。
