College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China.
College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China.
Talanta. 2025 Jan 1;281:126915. doi: 10.1016/j.talanta.2024.126915. Epub 2024 Sep 18.
Some biomarkers of acute aortic dissection (AAD) can be used for the potential supplementary diagnosis of AAD, such as C-reactive protein (CRP), smooth muscle myosin heavy chain (SmMHC), and D-dimer (D-D). However, the current measurement methods for common markers primarily rely on sophisticated instruments. The operation process is complicated, and the reagents used are expensive. To provide chronic disease monitoring and home self-examination services for potential AAD patients in real time, we developed a smartphone-based multichannel magnetoelastic (ME) immunosensing device to detect protein levels. Our immunosensor reduced the aforementioned restrictions and demonstrated excellent performance for the supplementary diagnosis of AAD. In this paper, we successfully combined the intelligent terminal with the hardware system to sample the resonance frequency shift (RFS) on the multichannel ME immunosensor. According to the target detection objects with their respective antibodies in the immune binding response, multiple experiments were conducted to detect multiple groups of samples, and we found that a CRP concentration, a SmMHC concentration, and a D-D concentration in the range of 0.1-100μg/mL, 1-4ng/mL, and 0.25-5μg/mL were linearly proportional to the RFS of the ME immunosensor, respectively. For CRP, SmMHC, and D-D, the sensitivities were 13.37Hz/μg∙mL, 155.19Hz/ng∙mL, and 332.72Hz/μg∙mL, respectively, and the detection limits were 2.634×10μg/mL, 1.155×10ng/mL, and 3.687×10μg/mL, respectively. The experiments demonstrated that the accuracy and stability of our device were comparable to those of the vector network analyzer (VNA, Calibration instrument).
一些急性主动脉夹层（AAD）的生物标志物可用于 AAD 的潜在辅助诊断，例如 C 反应蛋白（CRP）、平滑肌肌球蛋白重链（SmMHC）和 D-二聚体（D-D）。然而，目前常见标志物的测量方法主要依赖于复杂的仪器。操作过程复杂，使用的试剂昂贵。为了实时为潜在的 AAD 患者提供慢性病监测和家庭自检服务，我们开发了一种基于智能手机的多通道磁弹性（ME）免疫传感器来检测蛋白质水平。我们的免疫传感器减少了上述限制，并在 AAD 的辅助诊断中表现出出色的性能。在本文中，我们成功地将智能终端与硬件系统相结合，对多通道 ME 免疫传感器上的共振频率位移（RFS）进行采样。根据免疫结合反应中各自抗体的目标检测对象，进行了多次实验以检测多组样本，我们发现 CRP 浓度、SmMHC 浓度和 D-D 浓度在 0.1-100μg/mL、1-4ng/mL 和 0.25-5μg/mL 的范围内与 ME 免疫传感器的 RFS 呈线性比例。对于 CRP、SmMHC 和 D-D，灵敏度分别为 13.37Hz/μg·mL、155.19Hz/ng·mL 和 332.72Hz/μg·mL，检测限分别为 2.634×10μg/mL、1.155×10ng/mL 和 3.687×10μg/mL。实验表明，我们的设备的准确性和稳定性与矢量网络分析仪（VNA，校准仪器）相当。
