Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China.
Lab Chip. 2023 Apr 12;23(8):2048-2056. doi: 10.1039/d2lc01065h.
-Reaction protein (CRP) is a marker of nonspecific immunity for vital signs and wound assessment, and it can be used to diagnose infections in clinical medicine. However, measuring CRP level currently requires hospital-based instruments, high-cost reagents, and a complex process, all of which have limited its full capabilities for self-detection, a growing trend in modern medicine. In this study, we developed a novel smartphone-based device using advanced methods of magnetoelastic immunosensing to mitigate these limitations. We combined a system-on-chip (SoC) hardware architecture with smartphone apps to realize the sampling of resonance frequency shift on magnetoelastic chips, which can determine the ultra-sensitivity to mass change caused by the binding of anti-CRP antibody and CRP. Through detecting a multi-group of samples, we found that the resonance frequency shift was linearly proportional to the CRP concentration in the range from 0.1 to 100 μg mL, with a sensitivity of 12.90 Hz μg mL and a detection limit of 2.349 × 10 μg mL. Meanwhile, compared with the large-scale instrument used in clinical settings, the performance of our device was stable and significantly more portable, rapid and cost-effective, offering excellent potential for modern home-based diagnosis.
-反应蛋白（CRP）是一种用于生命体征和伤口评估的非特异性免疫标志物，可用于临床医学中感染的诊断。然而，目前测量 CRP 水平需要基于医院的仪器、高成本的试剂和复杂的过程，所有这些都限制了其在自我检测方面的全部功能，这是现代医学的一个日益增长的趋势。在这项研究中，我们开发了一种基于智能手机的新型设备，该设备使用先进的磁弹性免疫传感方法来缓解这些限制。我们将片上系统（SoC）硬件架构与智能手机应用程序相结合，实现了对磁弹性芯片上共振频率变化的采样，该变化可以确定抗 CRP 抗体和 CRP 结合引起的质量变化的超高灵敏度。通过检测多组样本，我们发现共振频率的偏移与 CRP 浓度在 0.1 到 100 μg mL 之间呈线性比例关系，灵敏度为 12.90 Hz μg mL，检测限为 2.349×10 μg mL。同时，与临床环境中使用的大型仪器相比，我们设备的性能更稳定，便携性、快速性和成本效益更高，为现代家庭诊断提供了出色的潜力。
