AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 3058565, Japan.
College of Life Science, Sichuan Normal University, Chengdu 610066, China.
Biomolecules. 2021 Oct 4;11(10):1454. doi: 10.3390/biom11101454.
(Ashwagandha) is used in Indian traditional medicine, Ayurveda, and is believed to have a variety of health-promoting effects. The molecular mechanisms and pathways underlying these effects have not yet been sufficiently explored. In this study, we investigated the effect of Ashwagandha extracts and their major withanolides (withaferin A and withanone) on muscle cell differentiation using C2C12 myoblasts. We found that withaferin A and withanone and Ashwagandha extracts possessing different ratios of these active ingredients have different effects on the differentiation of C2C12. Withanone and withanone-rich extracts caused stronger differentiation of myoblasts to myotubes, deaggregation of heat- and metal-stress-induced aggregated proteins, and activation of hypoxia and autophagy pathways. Of note, the Parkinson's disease model of Drosophila that possess a neuromuscular disorder showed improvement in their flight and climbing activity, suggesting the potential of Ashwagandha withanolides for the management of muscle repair and activity.
（印度人参）在印度传统医学阿育吠陀中被使用，被认为具有多种促进健康的作用。这些作用的分子机制和途径尚未得到充分探索。在这项研究中，我们使用 C2C12 成肌细胞研究了印度人参提取物及其主要的甾体生物碱（醉茄内酯 A 和醉茄酮）对肌肉细胞分化的影响。我们发现，醉茄内酯 A 和醉茄酮以及具有不同这些活性成分比例的印度人参提取物对 C2C12 的分化有不同的影响。醉茄酮和富含醉茄酮的提取物导致肌母细胞向肌管更强的分化，热和金属应激诱导的聚集蛋白的解聚，以及缺氧和自噬途径的激活。值得注意的是，具有神经肌肉障碍的果蝇帕金森病模型显示其飞行和攀爬活动能力的改善，表明印度人参甾体生物碱具有管理肌肉修复和活动的潜力。
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