Shi Shasha, Puzakov Mikhail, Guan Zhongxia, Xiang Kuilin, Diaby Mohamed, Wang Yali, Wang Saisai, Song Chengyi, Gao Bo
College of Animal Science & Technology, Yangzhou University, Yangzhou 225009, China.
A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov av., 2, 299011 Sevastopol, Russia.
Biology (Basel). 2021 Oct 7;10(10):1005. doi: 10.3390/biology10101005.
Here, a new superfamily of () DNA transposons, termed Sailor, is identified, that is characterized by a DD82E catalytic domain and is distinct from all previously known superfamilies of the group. Phylogenetic analyses revealed that forms a monophyletic clade with a more intimate link to the clades of and . was detected in both prokaryotes and eukaryotes and invaded a total of 256 species across six kingdoms. is present in nine species of bacteria, two species of plantae, four species of protozoa, 23 species of Chromista, 12 species of Fungi and 206 species of animals. Moreover, is extensively distributed in invertebrates (a total of 206 species from six phyla) but is absent in vertebrates. transposons are 1.38-6.98 kb in total length and encoded transposases of ~676 aa flanked by TIRs with lengths between 18, 1362 and 4 bp (TATA) target-site duplications. Furthermore, our analysis provided strong evidence of transmissions from prokaryotes to eukaryotes and internal transmissions in both. These data update the classification of the group and will contribute to the understanding of the evolution of transposons and that of their hosts.
在这里，我们鉴定出了一个新的（）DNA转座子超家族，称为水手（Sailor），其特征是具有DD82E催化结构域，并且与该组中所有先前已知的超家族不同。系统发育分析表明，（）与（）和（）的进化枝形成了一个单系进化枝，联系更为紧密。在原核生物和真核生物中均检测到（），它总共侵入了六个王国的256个物种。（）存在于9种细菌、2种植物、4种原生动物、23种色素界生物、12种真菌和206种动物中。此外，（）广泛分布于无脊椎动物中（来自六个门的总共206个物种），但在脊椎动物中不存在。（）转座子的总长度为1.38 - 6.98 kb，编码约676个氨基酸的转座酶，两侧是长度在18、1362和4 bp（TATA）之间的靶位点重复序列的末端反向重复序列（TIR）。此外，我们的分析提供了强有力的证据，证明（）从原核生物向真核生物的转移以及两者内部的转移。这些数据更新了该组的分类，并将有助于理解（）转座子及其宿主的进化。
