Kojima Kenji K, Bao Weidong
Genetic Information Research Institute, Cupertino, CA 95014, USA.
Biology (Basel). 2023 Feb 25;12(3):365. doi: 10.3390/biology12030365.
DDD/E transposase gene is the most abundant gene in nature and many DNA transposons in all three domains of life use it for their transposition. A substantial number of eukaryotic DNA transposons show similarity to prokaryotic insertion sequences (ISs). The presence of IS-like DNA transposons was indicated in the genome of . Here, we surveyed IS-like eukaryotic sequences using a bioinformatics approach and report a group of eukaryotic IS-like DNA transposons, designated , from parabasalids including . The lengths of target site duplications (TSDs) of are around 4 bps, around 15 bps, or around 25 bps, and strikingly, these discrete lengths of TSDs can be observed even in a single family. Phylogenetic analysis indicated the close relationships of with some of the prokaryotic IS family members. was not well separated from / in the phylogenetic analysis, but was distinct from other eukaryotic DNA transposons including and . The unique characteristics of in protein sequences and the distribution of TSD lengths support its placement as a new superfamily of eukaryotic DNA transposons.
DDD/E转座酶基因是自然界中最为丰富的基因，生命三个域中的许多DNA转座子都利用它进行转座。大量真核生物DNA转座子与原核生物插入序列（ISs）具有相似性。在……的基因组中发现了类似IS的DNA转座子。在此，我们使用生物信息学方法对类似IS的真核生物序列进行了调查，并报告了一组来自副基体（包括……）的类似真核生物IS的DNA转座子，命名为……。……的靶位点重复序列（TSD）长度约为4个碱基对、约15个碱基对或约25个碱基对，而且令人惊讶的是，即使在单个……家族中也能观察到这些不同长度的TSD。系统发育分析表明……与一些原核生物IS家族成员关系密切。在系统发育分析中，……与……/……没有很好地分开，但与包括……和……在内的其他真核生物DNA转座子不同。……在蛋白质序列上的独特特征以及TSD长度的分布支持将其归为真核生物DNA转座子的一个新超家族。
