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可可(L.)种子衍生肽通过与血管紧张素转换酶的催化位点相互作用降低血压。

Cocoa ( L.) Seed-Derived Peptides Reduce Blood Pressure by Interacting with the Catalytic Site of the Angiotensin-Converting Enzyme.

作者信息

Coronado-Cáceres Luis Jorge, Hernández-Ledesma Blanca, Mojica Luis, Quevedo-Corona Lucía, Rabadán-Chávez Griselda, Castillo-Herrera Gustavo Adolfo, Lugo Cervantes Eugenia

机构信息

Unidad de Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Guadalajara 44270, Mexico.

Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI UAM+CSIC), 28049 Madrid, Spain.

出版信息

Foods. 2021 Sep 30;10(10):2340. doi: 10.3390/foods10102340.

Abstract

This study aimed at determining the effect of cocoa proteins (CP) on the blood pressure, using in silico, in vitro and in vivo approaches. The in silico assay showed 26 Criollo cocoa peptides with alignment in the Blast analysis. Peptide sequences ranged from 6 to 16 amino acids, with molecular weight ranging from 560.31 to 1548.76 Da. The peptide sequences LSPGGAAV, TSVSGAGGPGAGR, and TLGNPAAAGPF showed the highest theoretical affinity with -8.6, -5.0, and -10.2 kcal/mol, for the angiotensin-converting enzyme (ACE), renin, and angiotensin II type 1 receptor (AT-R), respectively. The Criollo CP hydrolysates (CPH) presented in vitro ACE inhibitory activity with an IC value of 0.49 mg/mL. Furthermore, the orogastric administration of 150 mg CP/kg/day in rats fed a high-fat (HF) diet (HF + CP group) showed a significant decrease in systolic blood pressure (SBP) by 5% ( < 0.001) and diastolic blood pressure (DBP) by 7% ( < 0.001) compared with the HF group. The human equivalent dose (HED) of CP for an adult (60 kg) is 1.45 g per day. These results suggest that the consumption of CP could reduce blood pressure by blocking ACE, and could be used as an ingredient in the elaboration of antihypertensive functional foods.

摘要

本研究旨在通过计算机模拟、体外和体内实验方法确定可可蛋白(CP)对血压的影响。计算机模拟分析显示,26种克里奥罗可可肽在Blast分析中具有序列比对。肽序列长度为6至16个氨基酸,分子量范围为560.31至1548.76 Da。肽序列LSPGGAAV、TSVSGAGGPGAGR和TLGNPAAAGPF分别对血管紧张素转换酶(ACE)、肾素和血管紧张素II 1型受体(AT-R)表现出最高的理论亲和力,分别为-8.6、-5.0和-10.2 kcal/mol。克里奥罗CP水解物(CPH)在体外具有ACE抑制活性,IC值为0.49 mg/mL。此外,在高脂饮食喂养的大鼠(HF + CP组)中,经口胃给予150 mg CP/kg/天,与HF组相比,收缩压(SBP)显著降低5%(<0.001),舒张压(DBP)显著降低7%(<0.001)。成人(60 kg)的CP人体等效剂量(HED)为每天1.45 g。这些结果表明,食用CP可通过阻断ACE来降低血压,并可作为制备抗高血压功能性食品的一种成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02bd/8534856/1ed2b20bae8a/foods-10-02340-g001.jpg

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