Osman Ali, El-Hadary Abdalla, Korish Aida A, AlNafea Haifa M, Alhakbany Manan A, Awad Awad A, Abdel-Hamid Mahmoud
Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.
Biochemistry Department, Faculty of Agriculture, Benha University, Benha 13736, Egypt.
Foods. 2021 Feb 20;10(2):468. doi: 10.3390/foods10020468.
Papain hydrolysis of camel whey protein (CWP) produced CWP hydrolysate (CWPH). Fractionation of CWPH by the size exclusion chromatography (SEC) generated fractions (i.e., SEC-F1 and SEC-F2). The angiotensin converting enzyme inhibitory activity (ACE-IA) and free radical scavenging actions were assessed for CWP, CWPH, SEC-F1, and SEC-F2. The SEC-F2 exerted the highest ACE-IA and scavenging activities, followed by CWPH. The protective effects of CWPH on thioacetamide (TAA)-induced toxicity were investigated in rats. The liver enzymes, protein profile, lipid profile, antioxidant enzyme activities, renal functions, and liver histopathological changes were assessed. Animals with TAA toxicity showed impaired hepatorenal functions, hyperlipidemia, and decreased antioxidant capacity. Treatment by CWPH counteracted the TAA-induced oxidative tissue damage as well as preserved the renal and liver functions, the antioxidative enzyme activities, and the lipid profile, compared to the untreated animals. The current findings demonstrate that the ACE-IA and antioxidative effects of CWPH and its SEC-F2 fraction are worth noting. In addition, the CWPH antioxidative properties counteracted the toxic hepatorenal dysfunctions. It is concluded that the hydrolysis of CWP generates a wide range of bioactive peptides with potent antihypertensive, antioxidant, and hepatorenal protective properties. This opens up new prospects for the therapeutic utilization of CWPH and its fractions in the treatment of oxidative stress-associated health problems, e.g., hypertension and hepatorenal failure.
木瓜蛋白酶水解骆驼乳清蛋白（CWP）产生了骆驼乳清蛋白水解物（CWPH）。通过尺寸排阻色谱法（SEC）对CWPH进行分级分离得到了不同级分（即SEC-F1和SEC-F2）。对CWP、CWPH、SEC-F1和SEC-F2的血管紧张素转换酶抑制活性（ACE-IA）和自由基清除作用进行了评估。SEC-F2表现出最高的ACE-IA和清除活性，其次是CWPH。研究了CWPH对硫代乙酰胺（TAA）诱导的大鼠毒性的保护作用。评估了肝脏酶、蛋白质谱、血脂谱、抗氧化酶活性、肾功能和肝脏组织病理学变化。具有TAA毒性的动物表现出肝肾功受损、高脂血症和抗氧化能力下降。与未处理的动物相比，CWPH处理可抵消TAA诱导的氧化组织损伤，并保留肾功能、肝功能、抗氧化酶活性和血脂谱。当前研究结果表明，CWPH及其SEC-F2级分的ACE-IA和抗氧化作用值得关注。此外，CWPH的抗氧化特性抵消了有毒的肝肾功能障碍。得出的结论是，CWP的水解产生了多种具有强大的降压、抗氧化和肝肾保护特性的生物活性肽。这为CWPH及其级分在治疗与氧化应激相关的健康问题（如高血压和肝肾衰竭）中的治疗应用开辟了新的前景。
