Aqawi Muna, Sionov Ronit Vogt, Gallily Ruth, Friedman Michael, Steinberg Doron
The Biofilm Research Laboratory, The Faculty of Dental Medicine, The Institute of Dental Sciences, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel.
The Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel.
Microorganisms. 2021 Sep 25;9(10):2031. doi: 10.3390/microorganisms9102031.
is a common cariogenic bacterium in the oral cavity involved in plaque formation. Previous studies showed that Cannabigerol (CBG) has bacteriostatic and bacteriocidic activity against . The aim of the present study was to study its effect on biofilm formation and dispersion. was cultivated in the presence of CBG, and the resulting biofilms were examined by CV staining, MTT assay, qPCR, biofilm tracer, optical profilometry, and SEM. Gene expression was determined by real-time qPCR, extracellular polysaccharide (EPS) production was determined by Congo Red, and reactive oxygen species (ROS) were determined using DCFH-DA. CBG prevented the biofilm formation of shown by reduced biofilm biomass, decreased biofilm thickness, less EPS production, reduced DNA content, diminished metabolic activity, and increased ROS levels. CBG altered the biofilm roughness profile, resulting in a smoother biofilm surface. When treating preformed biofilms, CBG reduced the metabolic activity of with a transient effect on the biomass. CBG reduced the expression of various genes involved in essential metabolic pathways related to the cariogenic properties of biofilms. Our data show that CBG has anti-biofilm activities against and might be a potential drug for preventive treatment of dental caries.
是口腔中参与菌斑形成的常见致龋菌。先前的研究表明，大麻二酚（CBG）对……具有抑菌和杀菌活性。本研究的目的是研究其对……生物膜形成和分散的影响。在CBG存在的情况下培养……，并通过结晶紫染色、MTT法、qPCR、生物膜示踪剂、光学轮廓仪和扫描电子显微镜检查所得生物膜。通过实时qPCR测定基因表达，通过刚果红测定细胞外多糖（EPS）产量，使用2',7'-二氯二氢荧光素二乙酸酯（DCFH-DA）测定活性氧（ROS）。CBG通过降低生物膜生物量、减小生物膜厚度、减少EPS产量、降低DNA含量、降低代谢活性和提高ROS水平，阻止了……生物膜的形成。CBG改变了生物膜粗糙度轮廓，使生物膜表面更光滑。在处理预先形成的生物膜时，CBG降低了……的代谢活性，并对生物量产生短暂影响。CBG降低了与……生物膜致龋特性相关的基本代谢途径中各种基因的表达。我们的数据表明，CBG对……具有抗生物膜活性，可能是预防龋齿治疗的潜在药物。
